| 大肠杆菌F18ac菌毛F亚单位基因的克隆与序列分析 |
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| 引用本文： | 成大荣,徐建生,孙怀昌,唐波,吕玲,曹军.大肠杆菌F18ac菌毛F亚单位基因的克隆与序列分析[J].动物医学进展,2004,25(4):98-101. |
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| 作者姓名： | 成大荣 徐建生 孙怀昌 唐波 吕玲 曹军 |
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| 作者单位： | 扬州大学畜牧兽医学院,江苏,扬州,225009;扬州大学畜牧兽医学院,江苏,扬州,225009;扬州大学畜牧兽医学院,江苏,扬州,225009;扬州大学畜牧兽医学院,江苏,扬州,225009;扬州大学畜牧兽医学院,江苏,扬州,225009;扬州大学畜牧兽医学院,江苏,扬州,225009 |
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| 摘 要： | 根据已经发表的F18ab菌毛F亚单位(FedF／ab)的基因(fedF／ab)，设计一对引物，利用PCR技术从表达F18ac菌毛的大肠杆菌2134P株、8199株、8813株中分别扩增到一段序列，并克隆至pGEM—T载体，获得重组质粒T8813F、T8199F、T2134PF。琼脂糖凝胶电泳、序列测定及分析表明，该3个序列大小均为903bp，与fedF／ab大小一致且具有较高的同源性(99．4％)，推导的FedF／ac氨基酸序列与FedF／ab同源性为98．3％。数据表明该实验所克隆的序列均为F18ac菌毛F亚单位(FedF／ac)的基因(fedF／ac)。
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| 关 键 词： | 大肠杆菌 菌毛 F18 ac F亚单位 基因 分析 |
| 文章编号： | 1007-5038(2004)04-0098-04 |
Cloning and Identification of the Minor Subunit fedF Gene of the Fimbriae F18ac of E.coli |
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| CHENG Da-rong,XU Jian-sheng,SUN Huai-chang,TANG Bo,LU Ling,CAO Jun.Cloning and Identification of the Minor Subunit fedF Gene of the Fimbriae F18ac of E.coli[J].Progress In Veterinary Medicine,2004,25(4):98-101. |
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| Authors: | CHENG Da-rong XU Jian-sheng SUN Huai-chang TANG Bo LU Ling CAO Jun |
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| Abstract: | A pair of primers were designed and synthesized according to fedF of fimbriae F18ab (fedF/ab), and the coordinate subunit genes of F18ac (fedF/ac) were amplificated from three different Escherichia coli strains, 2134P?8199 and 8813, which are all display fimbriae F18ac. The PCR products of fedF/ac were cloned into pGEM-T vector. Color screening, PCR and restriction endonucleases analysis were used to identify the recombinant plasmids, and three recombinant plasmids, T8813F, T8199F and T2134PF were obtained. The three recombinant plasmids sequences were analyzed (with the MicroGenic computer program), and compared with the published sequences of fedF/ab. The data of sequences in the three fedF/ac genes showed that their sizes were in accord with the sequences of fedF/ab, and all shared 99.4% and 98.3% identity at nucleoide and amio acid level,respectively. All the data in this study conformed the three genes derived from the three different Escherichia coli strains, 2134P, 8199 and 8813, are all fedF/ac genes of F18ac. |
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| Keywords: | Escherichia coli fimbriae F18ac fedF/ac gene analysis |
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