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嫁接对茄子根际土壤微生物和叶片硝酸还原酶的影响
引用本文:周宝利,尹玉玲,徐妍,韩璐,叶雪凌,白丽萍.嫁接对茄子根际土壤微生物和叶片硝酸还原酶的影响[J].园艺学报,2010,37(1):53-58.
作者姓名:周宝利  尹玉玲  徐妍  韩璐  叶雪凌  白丽萍
作者单位:(沈阳农业大学园艺学院, 沈阳110866; 沈阳农业大学生物科学技术学院, 沈阳110866)
基金项目:国家自然科学基金项目(30771469); 国家科技支撑计划重点项目(2008BADA6B02); 辽宁省教育厅高等学校创新团队项目(2006T118)
摘    要:以野生茄‘托鲁巴姆’ (Solanum torvum ) 为砧木, 茄子(S. m elongena) ‘西安绿茄’品种为接穗, 研究了嫁接茄根际土壤微生物生理类群和土壤水解酶活性及叶片硝酸还原酶活性的动态变化特点,分析了嫁接茄根际土壤微生物生理类群和土壤水解酶与叶片硝酸还原酶活性的关系, 探讨了嫁接提高茄子氮素转化和吸收与土壤生物活性的关系。结果表明: 嫁接茄根际土壤微生物生理类群和土壤水解酶活性均较自根茄有增加的趋势。但不同发育时期也有一定的变化。嫁接茄叶片硝酸还原酶活性随着生育均表现出逐渐增加的趋势, 且显著高于自根处理。嫁接茄的固氮菌、硝化细菌和脲酶与其叶片硝酸还原酶活性均呈显著或极显著正相关。总体上看, 嫁接显著增加了茄子根际土壤固氮菌、硝化细菌和脲酶活性, 嫁接茄株也表现出较高的硝酸还原酶活性。

关 键 词:嫁接  茄子  硝酸还原酶  根际微生物  土壤酶  
收稿时间:2009-7-21
修稿时间:2009-12-28

Effect of Grafting on Rhizospheric Soil Microorganisms and Leaf NR Activities of Eggplants
ZHOU Bao-li,YIN Yu-ling,XU Yan,HAN Lu,YE Xue-ling,BAI Li-ping.Effect of Grafting on Rhizospheric Soil Microorganisms and Leaf NR Activities of Eggplants[J].Acta Horticulturae Sinica,2010,37(1):53-58.
Authors:ZHOU Bao-li  YIN Yu-ling  XU Yan  HAN Lu  YE Xue-ling  BAI Li-ping
Institution:(;1.College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China;2.College of B iological Science and Technology, Shenyang Agricultural University, Shenyang 110161, China)
Abstract:The commonly grown eggplant (Solanum melongena L.) cultivar Xi'an Luqie was used as scion. It was grafted with a wild eggplant rootstock (Solanum torvum). Dynamic characteristics in microbe physiological groups, hydrolase activity in rhizopheric soil and nitrate reductase (NR) activity of grafted eggplant leaves were studied. The relationships between microbial physiological groups, hydrolase activity in rhizopheric soil and leaves nitrate reductase (NR) activity were indicated. The relationship between nitrogen absorption and soil biological activities of grafted eggplants was discussed. Results showed that rhizopheric soil microbical physiological groups and hydrolase activity in grafted eggplants were higher in grafted eggplants than in self-rooted eggplants. However, these trends changed with different plant ages. The Leaves NR activity was increased with the plants ages and significant higher in grafted eggplants than in self-rooted eggplants. There were significant positive correlations between azotobacteria, nitrifying bacteria and urease activity in grafted eggplants. In conclusion, grafting increased the numbers of azotobacteria and nitrifying bacteria, urease activity in rhizopheric soil and NR in eggplant leaves.
Keywords:grafting  eggplants  NR  rhizospheric microbia  soil enzyme  
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