Hybrid aspen with a transgene for fungal manganese peroxidase is a potential contributor to phytoremediation of the environment contaminated with bisphenol A |
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| Authors: | Yosuke Iimura Madoka Yoshizumi Tomonori Sonoki Mikiko Uesugi Kenji Tatsumi Ken-ichi Horiuchi Shinya Kajita Yoshihiro Katayama |
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| Institution: | (1) Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8569, Japan;(2) Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan;(3) Core Project, Technical Center, Nitta Corporation, Yamatokoriyama 639-1085, Japan |
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| Abstract: | To assess the possible utility of a fungal gene for manganese-dependent peroxidase (MnP) produced by a transgenic plant in
phytoremediation, we transformed hybrid aspen with a chimeric gene for MnP. Our gene construct allowed expression of the gene
for MnP in plants and relatively high MnP activity was detected in the hydroponic medium in which roots of plants that expressed
the transgene had been cultured. Some of our transgenic plants were able to remove bisphenol A from the medium more efficiently
than wild-type plants. Our results demonstrate that, without any modification of the coding sequence, a chimeric gene for
fungal MnP can be expressed in a woody plant, with secretion of active MnP from roots into the rhizosphere. Our strategy suggests
new options using woody plants for phytoremediation. |
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| Keywords: | Bisphenol A Manganese-dependent peroxidase Phytoremediation Populus Trametes versicolor |
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