Characterization of an endopolygalacturonase Gene cppg1 from Phytopathogenic Fungus Chondrostereum purpureum |
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| Authors: | Mineo SENDA Takanori NARITA Shinji AKADA Toshikatsu OKUNO Kazuo MIYAIRI |
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| Institution: | (1) Gene Research Center, Hirosaki University, Hirosaki 036–8561, Japan, JP;(2) Laboratory of Biochemistry, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036–8561, Japan, JP |
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| Abstract: | Chondrostereum purpureum, a phytopathogenic fungus, produces endopolygalacturonase (endoPG) which has been suggested to have a causal role in the silver-leaf symptom of apple trees. In this paper, we detected C. purpureurn-derived endoPG at the infection sites using ELISA with a polyclonal antibody against endoPG I. A gene encoding endoPG I and its homolog were also isolated from the C. purpureum genome. The endoPG I gene was designated as cppg1. The cppg1 gene is the first fungal endoPG gene reported in the Basidiomycetes.
Received 31 May 2000/ Accepted in revised form 13 September 2000 |
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| Keywords: | : Chondrostereum purpureum apple silver-leaf disease endopolygalacturonase ELISA gene |
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