| 埃博拉病毒抗体间接血凝检测方法的建立 |
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| 引用本文： | 张颖,曹增国,吴芳芳,李国华,李恩涛,焦翠翠,迟航,金宏丽,韩秋雪,张胜男,王化磊,冯娜,高玉伟,赵永坤,杨松涛,夏咸柱.埃博拉病毒抗体间接血凝检测方法的建立[J].中国兽医学报,2019(4):688-694. |
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| 作者姓名： | 张颖 曹增国 吴芳芳 李国华 李恩涛 焦翠翠 迟航 金宏丽 韩秋雪 张胜男 王化磊 冯娜 高玉伟 赵永坤 杨松涛 夏咸柱 |
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| 作者单位： | 1.吉林农业大学动物科学技术学院;2.军事科学院军事医学研究院军事兽医研究所/吉林省人兽共患病预防与控制重点实验室;3.吉林大学动物医学学院;4.石河子大学动物科技学院 |
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| 基金项目： | 国家科技重大专项(重大新药创制)资助项目(2015ZX09102025) |
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| 摘 要： | 以纯化后的埃博拉病毒(Ebola virus,EBOV)糖蛋白(GP)作为抗原,致敏醛化的绵羊红细胞,进行最佳反应条件优化,建立间接血凝抗体检测方法。以EBOV高免马血清、纯化的IgG和精制免疫球蛋白F(ab’)_2为待检样品进行检测,并将检测结果与假病毒中和试验检测结果相比较。结果显示,该方法可特异性检测EBOV抗体,与马尔堡病毒、裂谷热病毒等的阳性血清均不发生反应;与假病毒中和试验测得的抗体效价增长规律相一致。结果表明,本试验成功建立了EBOV抗体间接血凝检测方法,该方法安全、简便、快捷,为EBOV疫苗免疫后的效果评价提供了又一新的检测方法。
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| 关 键 词： | 埃博拉病毒 糖蛋白(GP) 间接血凝试验 抗体检测 |
Development of an indirect hemagglutination for detecting Ebola virus antibody |
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| ZHANG Ying,CAO Zeng-guo,WU Fang-fang,Guo-hua,LI En-tao,JIAO Cui-cui,CHI Hang,JIN Hong-li,HAN Qiu-xue,ZHANG Sheng-nan,WANG Hua-lei,FENG Na,GAO Yu-wei,ZHAO Yong-kun,YANG Song-tao,XIA Xian-zhu.Development of an indirect hemagglutination for detecting Ebola virus antibody[J].Chinese Journal of Veterinary Science,2019(4):688-694. |
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| Authors: | ZHANG Ying CAO Zeng-guo WU Fang-fang Guo-hua LI En-tao JIAO Cui-cui CHI Hang JIN Hong-li HAN Qiu-xue ZHANG Sheng-nan WANG Hua-lei FENG Na GAO Yu-wei ZHAO Yong-kun YANG Song-tao XIA Xian-zhu |
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| Institution: | (College o f Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;histitute of Military Veterinary Medici tie , Academy of Military Medical Sciences , Academy o f Military Sciences ,Changchun 130122 <, Ch i?ia;College o f Veterinary Medicine J ilin University,Changchun 130062,China;College of Animal Scietice and Technology , Shihezi University , Shihezi , Xinjiang 832003, China;College of Veterinary Medicine , South Ch ina Agricultural University , Guangzhou 510000China;College o f Atiimat Science and Technology , Northeast Agricultural University Harbin 150030 China;College of Wildlife Resources , Northeast Forestry University .Harbin 150030,China) |
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| Abstract: | ZHANG Ying;CAO Zeng-guo;WU Fang-fang;Guo-hua;LI En-tao;JIAO Cui-cui;CHI Hang;JIN Hong-li;HAN Qiu-xue;ZHANG Sheng-nan;WANG Hua-lei;FENG Na;GAO Yu-wei;ZHAO Yong-kun;YANG Song-tao;XIA Xian-zhu(College o f Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;histitute of Military Veterinary Medici tie , Academy of Military Medical Sciences , Academy o f Military Sciences ,Changchun 130122 <, Ch i?ia;College o f Veterinary Medicine J ilin University,Changchun 130062,China;College of Animal Scietice and Technology , Shihezi University , Shihezi , Xinjiang 832003, China;College of Veterinary Medicine , South Ch ina Agricultural University , Guangzhou 510000China;College o f Atiimat Science and Technology , Northeast Agricultural University Harbin 150030 China;College of Wildlife Resources , Northeast Forestry University .Harbin 150030,China) |
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| Keywords: | Ebola virus envelope glycoprotein (GP) indirect hemagglutination antibody detection |
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