| Q型烟粉虱芳香基硫酸酯酶B基因的克隆及表达分析 |
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| 引用本文： | 宋天雪,田丽霞,谢文,崔洪莹,向文胜,张友军.Q型烟粉虱芳香基硫酸酯酶B基因的克隆及表达分析[J].植物保护学报,2019,46(1):40-48. |
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| 作者姓名： | 宋天雪 田丽霞 谢文 崔洪莹 向文胜 张友军 |
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| 作者单位： | 东北农业大学生命科学学院, 哈尔滨 150030,中国农业科学院蔬菜花卉研究所, 北京 100081,中国农业科学院蔬菜花卉研究所, 北京 100081,中国农业大学植物保护学院, 北京 100193,东北农业大学生命科学学院, 哈尔滨 150030,中国农业科学院蔬菜花卉研究所, 北京 100081 |
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| 基金项目： | 国家自然科学基金（31601637，31572014），国家重点研发计划（2017YFD0201000） |
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| 摘 要： | 为明确烟粉虱Bemisia tabaci取食感染番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的番茄植株后,其体内的芳香基硫酸酯酶B基因(arylsulfatase B,ARSB)是否能够做出应答反应,基于Q型烟粉虱基因组数据克隆得到ARSB基因cDNA全长,采用生物信息学方法分析其序列特征,并通过实时荧光定量PCR技术测定ARSB基因在Q型烟粉虱不同发育阶段、不同组织及携带TYLCV前后的表达量变化情况。结果显示:Q型烟粉虱ARSB基因的cDNA全长为1 731 bp,编码576个氨基酸,分子量为64.89 kD,具有ARSB的保守结构域。ARSB基因在Q型烟粉虱不同发育阶段均有表达,在卵期表达量最高,成虫期表达量最低;该基因在Q型烟粉虱头胸部的表达量显著高于腹部;Q型烟粉虱获取TYLCV 72 h后其体内ARSB基因表达量显著提高。表明ARSB基因在Q型烟粉虱不同龄期、不同组织内存在差异表达,并可能参与Q型烟粉虱对TYLCV的响应和传毒过程。
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| 关 键 词： | Q型烟粉虱 芳香基硫酸酯酶B 基因克隆 表达分析 |
| 收稿时间： | 2018/7/9 0:00:00 |
Molecular cloning and expression analysis of the arylsulfatase B gene in Bemisia tabaci Q biotype |
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| Song Tianxue,Tian Lixi,Xie Wen,Cui Hongying,Xiang Wensheng and Zhang Youjun.Molecular cloning and expression analysis of the arylsulfatase B gene in Bemisia tabaci Q biotype[J].Acta Phytophylacica Sinica,2019,46(1):40-48. |
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| Authors: | Song Tianxue Tian Lixi Xie Wen Cui Hongying Xiang Wensheng and Zhang Youjun |
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| Institution: | College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China,Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China,Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China,College of Plant Protection, China Agricultural University, Beijing 100193, China,College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China and Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | In order to investigate whether the arylsulfatase B (ARSB) gene of Bemisia tabaci Q biotype can respond to Tomato yellow leaf curl virus (TYLCV) on tomatoes, the full-length cDNA of ARSB gene was cloned from B. tabaci Q biotype based on the genomic data; the sequence characteristics of the gene were analyzed with bioinformatics, and the expression levels of ARSB gene in different developmental stages, different tissues and under TYLCV treatment were detected in B. tabaci Q biotype by using quantitative real-time PCR. The results showed that the cDNA length of ARSB gene was 1 731 bp, encoding 576 amino acids, with a molecular weight of 64.89 kD. It had the conserved domain of ARSB. The ARSB gene was expressed during different development stages of B. tabaci Q biotype. The expression level was the highest in the egg stage and the lowest in the adult stage. The expression level of ARSB gene in the head and thorax was significantly higher than that in the abdomen, and the expression level of ARSB gene under TYLCV treatment for 72 h was significantly higher than that without TYLCV treatment. This study indicated that the expression level of ARSB varied at different developmental stages and in different tissues of B. tabaci Q biotype. The ARSB gene might play a potential role in the response of B. tabaci Q biotype to TYLCV. |
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| Keywords: | Bemisia tabaci Q biotype arylsulfatase B gene cloning expression analysis |
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