| 铅胁迫对茶条槭与五角槭的叶片叶绿素含量和膜脂过氧化及保护酶活性的影响 |
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| 引用本文： | 李亚藏.铅胁迫对茶条槭与五角槭的叶片叶绿素含量和膜脂过氧化及保护酶活性的影响[J].湖南农业大学学报(自然科学版),2012,38(4):404-407. |
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| 作者姓名： | 李亚藏 |
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| 作者单位： | 安阳工学院土木与建筑工程学院,河南安阳455000 |
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| 基金项目： | 国家自然科学基金项目(30371149);黑龙江省科技计划项目(GCO1KBKB213) |
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| 摘 要： | 采用盆栽方法,研究茶条槭(Acer ginnala Maxim)和五角槭(Acer mono Maxim)一年生苗木对铅胁迫的耐受情况。在栽培基质中加入Pb(CH3COO)2.5H2O试剂(分析纯),配制成Pb2+质量浓度分别为100、500、1 000、2 000mg/kg的基质,并设1个对照处理。苗木生长75 d以后,取叶样测定各项指标。试验结果:随着土壤Pb2+浓度的增加,两树种叶片中叶绿素a含量(茶条槭除外)、叶绿素b含量、叶绿素总量及脯氨酸含量降低,相对电导率、丙二醛(MDA)含量和过氧化物酶(POD)活性升高,超氧化物歧化酶(SOD)活性先上升后下降,上述指标的变化幅度均表现为五角槭大于茶条槭。结果表明:铅胁迫导致植物体内发生了膜脂过氧化反应,且反应随胁迫强度的增加而增加,同时引发抗氧化反应;在清除过氧化物过程中,SOD发挥了主要作用,POD活性变化不明显。综合比较,两树种对铅胁迫均具有一定的耐受性,茶条槭的耐受性比五角槭的大,两树种均可用作铅胁迫环境下的城市绿化树种。
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| 关 键 词： | 茶条槭 五角槭 铅胁迫 叶绿素 膜质过氧化 抗氧化酶 脯氨酸 |
| 收稿时间： | 2012/4/18 0:00:00 |
| 修稿时间： | 2012/4/18 0:00:00 |
Effect of lead stress on chlorophyll content, membrane lipid peroxidation and protectiveenzyme activity in leaves of Acer ginnala Maxim and Acer mono Maxim |
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| liyacang.Effect of lead stress on chlorophyll content, membrane lipid peroxidation and protectiveenzyme activity in leaves of Acer ginnala Maxim and Acer mono Maxim[J].Journal of Hunan Agricultural University,2012,38(4):404-407. |
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| Authors: | liyacang |
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| Institution: | LI Ya-cang(College of Civil and Building Engineering College,Anyang Institute of Technology,Anyang,Henan 455000,China) |
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| Abstract: | The membrane lipid peroxidation and protective enzyme activity in the annual seedlings of Acer ginnala Maxim and Acer mono Maxim were studied under lead stress through pot experiment.Treatments Pb100,Pb500,Pb1000,Pb2000 were added with 100,500,1 000 and 2 000 mg/kg of Pb(CH3COO)2?5H2O(pure Pb2+) respectively and the treatment with no Pb2+ addition was served as control(CK).Indexes of the leaf samples were determined 75 d after Pb addition.The results showed that chlorophyll a/b and proline contents decreased while relative conductivity,manlondialdehyde(MDA) content and the activity of peroxidase(POD) increased with the increase of lead concentration in soil.The activity of superoxide dismutase(SOD) rose firstly and then went down.The variations of these indexes in Acer mono were greater than those in Acer ginnala.Lead stress caused membrane lipid peroxidation in the plants and the peroxidation increased with increasing intensity of lead stress,which meanwhile caused anti-peroxidation effect.SOD played an important role and the change of POD was not obvious during the process of removing peroxidation products.In general,both Acer ginnala Maxim and Acer mono Maxim were tolerant to lead stress and the former had greater tolerance than the latter.Two kinds of trees could be used as landscape trees in lead polluted area. |
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| Keywords: | Acer ginnala Maxim Acer mono Maxim lead stress chlorophyll membrane lipid peroxidation antioxidant enzyme proline |
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