| 木本植物种质资源超低温保存研究进展 |
| |
| 引用本文： | 熊兴耀,李炎林.木本植物种质资源超低温保存研究进展[J].湖南农业大学学报(自然科学版),2012,38(4):347-353. |
| |
| 作者姓名： | 熊兴耀 李炎林 |
| |
| 作者单位： | 1. 中国农业科学研究院蔬菜花卉研究所,北京100081
2. 湖南农业大学园艺园林学院,湖南长沙410128 |
| |
| 基金项目： | 国家教育部项目(IRT0963) |
| |
| 摘 要： | 超低温保存是目前木本植物长期保存的理想方法。综述国内外超低温保存技术在木本植物种质资源保存方面的应用现状和超低温条件下的低温伤害及细胞超微结构改变,阐述超低温保存技术的发展及各类超低温保存技术的优缺点和影响因素,指出广谱适用性问题、保存方法的可重复性问题和可操作性问题、投入产出问题是影响超低温保存技术推广应用的主要问题,认为扩大超低温保存木本植物资源的种类、根据植物种类和外植体类型发展相应的超低温保存技术、根据保存目的合理选取保存材料及其保存方法、优先发展经济优势物种的保存技术、增加财政投入以研发新型超低温保存技术是解决上述问题的重要途径。
|
| 关 键 词： | 木本植物 种质资源 超低温保存 低温伤害 |
| 收稿时间： | 2012/5/10 0:00:00 |
| 修稿时间： | 2012/5/10 0:00:00 |
Progress in cryopreservation of woody plant germplasm |
| |
| XIONG Xing-yao,LI Yan-lin.Progress in cryopreservation of woody plant germplasm[J].Journal of Hunan Agricultural University,2012,38(4):347-353. |
| |
| Authors: | XIONG Xing-yao LI Yan-lin |
| |
| Institution: | 1.The Institute of Vegetables and Flowers,Chinese Academy of Agriculture Sciences,Beijing 100081,China;2.College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China) |
| |
| Abstract: | Cryopreservation is considered to be a valuable solution for long-term storage of plant germplasm especially for woody plant species.In this article,we summarized the application of cryopreservation in woody plant species,the damage and ultrastructural changes of freezing cell and the development of various cryopreservation techniques and their advantages and disadvantages.We pointed out that the spectrum,repeatability and maneuverability of cryopreservation plus high investment and low output of this technique were main problems existed at present.To solve these problems,various suggestions were put forward,such as expanding the woody plant resources for cryopreservation,developing cryopreservation techniques according to plant species,choosing the right conservation materials and methods based on the purpose of preservation,giving priority to development of preservation techniques for economic species and raising investment for developing new cryopreservation methods. |
| |
| Keywords: | woody plant species germplasm resources cryopreservation low-temperature damage |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《湖南农业大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《湖南农业大学学报(自然科学版)》下载免费的PDF全文 |
|
