| 一株吡嘧磺隆降解菌DF12的筛选、鉴定和降解特性研究 |
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| 引用本文： | 陈颖曦,柏连阳,曾爱平,周小毛,宋杰辉,王彦辉,刘二明.一株吡嘧磺隆降解菌DF12的筛选、鉴定和降解特性研究[J].湖南农业大学学报(自然科学版),2012,38(3):287-290. |
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| 作者姓名： | 陈颖曦 柏连阳 曾爱平 周小毛 宋杰辉 王彦辉 刘二明 |
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| 作者单位： | 湖南农业大学 生物安全科学技术学院,湖南 长沙,410128 |
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| 基金项目： | 国家烟草专卖局项目，湖南省研究生创新基金项目 |
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| 摘 要： | 从湖南瑞泽农药厂污水池中筛选到1株吡嘧磺隆降解菌DF12,经形态学、生理生化及16S rDNA鉴定为恶臭假单胞菌(Pseudomonas putida),该菌在100 mL三角瓶中摇瓶体积70 mL、接种量2.0%(OD600=0.80)、pH7.0条件下对吡嘧磺隆降解率可达74.78%。
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| 关 键 词： | 吡嘧磺隆 假单胞菌属 降解特性 |
| 收稿时间： | 2012/1/9 0:00:00 |
| 修稿时间： | 2012/2/20 0:00:00 |
Isolation, Identification and Characterization of a Pyrazosulfuronethyl Degradating Strain DF12 |
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| ChenYingxi,and.Isolation, Identification and Characterization of a Pyrazosulfuronethyl Degradating Strain DF12[J].Journal of Hunan Agricultural University,2012,38(3):287-290. |
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| Authors: | ChenYingxi and |
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| Institution: | (College of Bio-Safety Science and Technology,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | A Pyrazosulfuronethyl-degrading strain DF12 was isolated from sewage reservoir of the herbicides factory in Hunan,which belonged to Pseudomonas putida based on physiological and biochemical properties and 16S rDNA analysis.When strain DF12 grown in LB medium was adjusted to an OD600 of 0.80 and inoculated in an inoculum size of 2.0% in 70 mL culture containing Pyrazosulfuronethyl in a 100 mL flask with shaking under pH of 7.0,it could degrade 74.78 % of Pyrazosulfuron-ethyl. |
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| Keywords: | Pyrazosulfuronethyl Pseudomonas sp degradating characters |
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