| 邓恩桉丛生芽的诱导 |
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| 引用本文： | 蒋淑磊,徐刚标.邓恩桉丛生芽的诱导[J].经济林研究,2008,26(2). |
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| 作者姓名： | 蒋淑磊 徐刚标 |
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| 作者单位： | 中南林业科技大学,湖南,长沙,410004 |
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| 基金项目： | 中南林业科技大学人才引进项目 |
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| 摘 要： | 为了建立优良邓恩桉无性系组织培养与再生体系,为该优良无性系的推广提供大规模的商品化苗木,并在此基础上,进一步建立桉树转基因系统,培育速生耐寒转基因桉树新品种,扩大其栽培区域,以湖南省植物园选育出的优良邓恩桉Eucalyptusdunnii无性系为材料,采取1年生苗上部带饱满腋芽的茎段作为外植体,诱导芽的分化及丛生芽的产生。通过不同质量浓度的生长素NAA和细胞分裂素6-BA的组合对比试验,确定了邓恩桉茎芽分化的最佳培养基为MS 6-BA 0.5 mg/L NAA 0.2 mg/L,芽继代增殖最佳培养基为改良MS 6-BA 0.3 mg/L NAA 0.05 mg/L。
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| 关 键 词： | 植物生理学 邓恩桉 组织培养 丛生芽 诱导 |
Induction of Multiple Buds from Stem Segments in Eucalyptus dunnii |
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| JIANG Shu-lei,XU Gang-biao.Induction of Multiple Buds from Stem Segments in Eucalyptus dunnii[J].Economic Forest Researches,2008,26(2). |
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| Authors: | JIANG Shu-lei XU Gang-biao |
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| Abstract: | In order to establish tissue culture and regeneration system of fine Eucalyptus dunnii clone selected out by Hunan Botanical Garden,to provide large-scale commercial seedlings for extending the clone,and to establish transgenic system and cultivate new fast-growing and hardy varieties,differentiation of buds and proliferation of multiple buds were induced from stem segments with an axillary bud,taking 1-year-old seedlings in E.dunnii as materials.The results showed that the better medium for differentiation of buds was MS supplemented with 6-BA 0.5 mg/L and NAA 0.2 mg/L,and the better medium for proliferation of multiple buds was MS supplemented with 6-BA 0.3 mg/L and NAA 0.05 mg/L. |
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| Keywords: | plant physiology Eucalytus dunnii tissue culture multiple buds induction |
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