Detection of a novel chloramphenicol resistance plasmid from "equine" Staphylococcus sciuri |
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| Authors: | S Schwarz M Cardoso H Blobel |
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| Institution: | Institut für Bakteriologie und Immunologie, Justus-Liebig-Universit?t Giessen, FRG. |
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| Abstract: | A small chloramphenicol resistance (Cm) plasmid of 4.65 kB could be detected in an "equine" Staphylococcus sciuri-culture. This plasmid, designated as pSC3, was identified by interspecific protoplast transformation. On the basis of restriction endonuclease analyses a detailed restriction map of pSC3 could be constructed. This allowed structural comparisons of pSC3 with Cm-plasmids of other staphylococcal species from infections of humans and animals and identification of pSC3 as a member of the pC 221-family of staphylococcal Cm-plasmids. The pSC3-plasmid encoded an inducible chloramphenicol acetyltransferase as confirmed by enzymatic assays. This enzyme could be demonstrated in cell-free lysates of Cm-induced pSC3-transformants. |
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