| 单抗间接Dot-ELISA检测禽脑脊髓炎病毒抗原的研究 |
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| 引用本文： | 秦卓明,杨建民,赵继勋,苏敬良.单抗间接Dot-ELISA检测禽脑脊髓炎病毒抗原的研究[J].畜牧与兽医,2002,34(3):8-11. |
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| 作者姓名： | 秦卓明 杨建民 赵继勋 苏敬良 |
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| 作者单位： | 1. 山东省家禽研究所,山东,济南,250100
2. 中国农业大学实验动物所,北京,100094 |
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| 基金项目： | 山东省农科院青年基金资助课题 (1 998 2 0 0 1 ) |
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| 摘 要： | 利用制备的禽脑脊髓炎病毒 (AEV)单抗建立了检测AEV抗原的间接Dot ELISA方法 ,对AEV最低检出量为 1 6 0 8μg/mL。人工感染 1日龄SPF鸡 ,取发病鸡 (6～1 3日龄 )相应病料 ,各病料样本阳性检出率分别为 :脑 1 0 0 % ,胰 96 % ,十二指肠 98% ,腺胃 98%。对 40份临床自然发病鸡病料检测 ,阳性检出率为 92 %。对 1 0份AEV病原分离阳性样品 ,检测阳性率为 1 0 0 % ,而SPF鸡对照均为阴性
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| 关 键 词： | 禽脑脊髓炎病毒抗原 单抗 间接DotELISA |
| 文章编号： | 0529-5130(2002)03-0008-04 |
Establishment of indirect Dot-ELISA with McAb for detection of AEV antigen |
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| QIN Zhuo ming ,YANG Jian min ,ZHAO Ji xun ,SU Jing liang.Establishment of indirect Dot-ELISA with McAb for detection of AEV antigen[J].Animal Husbandry & Veterinary Medicine,2002,34(3):8-11. |
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| Authors: | QIN Zhuo ming YANG Jian min ZHAO Ji xun SU Jing liang |
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| Institution: | QIN Zhuo ming 1,YANG Jian min 2,ZHAO Ji xun 2,SU Jing liang 2 |
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| Abstract: | The procedure of indirect Dot ELISA has been successfully established for detection of AEV antigen,and the detection limit of the AEV was 16 08 μg/mL,The one day old SPF chickens were infected with AEV(V R and Shandong isolates)by the way of brains and subcutaneous in neck jection,and the pathological tissues and organs infected were collected when they are 6～13 day old.It was shown that the positive rates of AEV antigen in brain,pancreas,duodenum,gland stomach are 100%,96%,98%,98%respectively.These organs are the major targets infected by AEV.Forty samples collected from field infected chickens having clinical signs were detected,and the positive rate of AEV was 92%. |
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| Keywords: | AEV monoclonal antibody indirect Dot ELISA |
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