| 乐果对大鼠肝细胞凋亡作用的研究 |
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| 引用本文： | 刘学忠,袁楷,袁燕,卞建春,刘宗平.乐果对大鼠肝细胞凋亡作用的研究[J].上海交通大学学报(农业科学版),2009,27(2). |
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| 作者姓名： | 刘学忠 袁楷 袁燕 卞建春 刘宗平 |
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| 作者单位： | 扬州大学,兽医学院,扬州,225009 |
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| 基金项目： | 江苏省高校青蓝工程中青年学术带头人培养对象资助项目，扬州大学科技创新培育基金 |
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| 摘 要： | 通过给大鼠肝细胞培养液中加入乐果(染毒终浓度分别为0、3、10、30、100和300 μmol·L-1),染毒12、24 h后,Annexin V/PI双染法检测肝细胞凋亡率;分别用Fluo-2/AM、双氢-乙酰乙酸二氯荧光黄(DCFH-DA)和罗丹名123检测细胞内Ca2+浓度、活性氧(ROS)和线粒体膜电位(△Ψm)变化,并在扫描电镜和荧光显微镜下观察凋亡细胞情况,研究乐果对大鼠肝细胞凋亡的影响.结果表明,肝细胞染毒12和24 h后,出现了明显的细胞凋亡的形态学变化,细胞凋亡率明显升高,除3 μmol·L-1组外,与对照组相比差异显著(P<0.05或P<0.01),且呈时间-剂量效应.3 μmol·L-1组细胞内Ca2+浓度极显著高于对照组(P<0.01),之后随染毒剂量的增加,细胞内Ca2+度逐渐下降;细胞内ROS水平在3-100 μmol·L-1范围内随染毒剂量的增大和染毒时间的延长而升高,而在300 μmol·L-1组略有下降,除3 μmol·L-1组外,与对照组相比均差异极显著(P<0.01);△Ψm除24h 300 μmol·L-1组外均出现持续下降.表明低剂量乐果染毒可诱导肝细胞发生凋亡,细胞内Ca2+、ROS和△Ψm可能参与了这一过程.
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| 关 键 词： | 乐果 凋亡 细胞内Ca2 浓度 活性氧 线粒体膜电位 mitochondrial membrane potential (△Ψm) |
Studies on the Effects of Dimethoate on Rat Hepatic cell Apoptosis |
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| LIU Xue-zhong,YUAN Kai,YUAN Yan,BIAN Jian-chun,LIU Zong-ping.Studies on the Effects of Dimethoate on Rat Hepatic cell Apoptosis[J].Journal of Shanghai Jiaotong University (Agricultural Science),2009,27(2). |
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| Authors: | LIU Xue-zhong YUAN Kai YUAN Yan BIAN Jian-chun LIU Zong-ping |
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| Abstract: | In order to study the effects of Dimethoate on liver cell apoptosia in vitro, Dimethoate was added to rat hepatocyte culture fluid served by electron and fluorescence microscope, apoptosis rate was analyzed by Annexin WPI method; Intracellular Ca2+ concentration, reactive oxygen species (ROS) and mitochondria membrane potential (△Ψm) changes were analyzed by Fluo-2/AM, Dichlorofluorescin di-acetate (DCFH-DA) and Rhodamine 123 method respectively. The results showed that after 12 and 24 hours' exposure, mierostructural changes of apoptotic cells were observed; hepatic cell apoptosis rate increased, and the difference between experimental groups (apart decreased with increasing dosage. Intracellular ROS level increased with increasing dosage and time of exposure in the range of 3～100 liver cell apoptosis, and intracellular Ca2+, ROS and △Ψm changes may participate in this process. |
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| Keywords: | Dimethoate apoptosis intracellular Ca2 concentration reactive oxygen species (ROS) |
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