| 海南岛热带雨林区芽孢杆菌收集及Bt菌鉴定 |
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| 引用本文： | 张文飞,全嘉新,谢柳,王茜,易艳桃,丰玫玫,朱麟,王锐萍,方宣钧.海南岛热带雨林区芽孢杆菌收集及Bt菌鉴定[J].广西农业生物科学,2009(2):265-274. |
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| 作者姓名： | 张文飞 全嘉新 谢柳 王茜 易艳桃 丰玫玫 朱麟 王锐萍 方宣钧 |
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| 作者单位： | 海南海德热带农业资源研究所;广西大学生命科学与技术学院;海南师范大学生命科学院; |
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| 基金项目： | 中国Bt资源收鉴与利用项目;;国家863项目(2006AA022189)资助 |
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| 摘 要： | 本研究系统地对海南岛热带雨林自然保护区进行了土壤样品的采集、芽孢杆菌的分离收集和Bt菌株的鉴定。从尖峰岭热带雨林区、五指山热带雨林区、吊罗山热带雨林区、霸王岭热带雨林区总共采集了土壤样品1882份，采用醋酸钠培养基结合高温方法分离出芽孢杆菌3924份，鉴定出Bt分离株158份，Bt菌株的分离率和出菌率分别为4.03％和8.40％。结果分析表明，海南岛热带雨林区芽孢杆菌及Bt菌株分布对环境和生态表现出一定的规律性，一般海拔900m至1400m的反菌株含量高、植被覆盖率高，土壤腐殖质含量高的热带沟谷雨林带眈菌株含量最高。显微观察发现，获得的Bt菌株其伴胞晶体有菱形、球形、方形、椭球形、不定性等多种形状。利用SDS-PAGE方法对获得的Bt分离株进行了伴胞晶体进行分析，发现伴胞晶体的分子量有20kD到150kD不等。进一步利用PCR-RFLP技术对Bt分离株进行了cry基因型的分析，初步发现这些Bt菌株含有cry1、cry3、cry4、cry6、cry30、cry40等基因型。我们还利用鳞翅目昆虫小菜蛾和鞘翅目昆虫椰心叶甲进行部分Bt分离株的生物测定，初步结果显示本研究鉴定出的Bt分离株具有不同的抗虫靶标，对同一靶标昆虫也表现出不同的杀虫活性。整体而言，本研究结果显示出海南岛热带热带雨林自然保护区因其独特的热带地理生境、自然的生物演化系统，使得热带雨林区蕴藏了Bt菌株资源多样化，值得期待挖掘出一些新的菌株和新的基因资源。
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| 关 键 词： | 芽孢杆菌 苏云金芽孢杆菌 伴胞晶体蛋白 cry基因型 海南岛热带雨林区 |
Collection of Bacillus and Identification of Bacillus thuringensis Isolates from Tropical Rain Forest Reserves of Hainan Island |
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| Zhang Wenfei , Quan Jiaxin Xie Liu , Wang Xi , Yi Yantao Feng Meimei Zhu Lin Wang Ruiping Fang Xuanjun Haide Institute of Tropical Agricultural Resources,Sanya, College of Life Science , Technology,Guangxi University,Nanning, College of Life Sciences,Hainan Normal University,Haikou.Collection of Bacillus and Identification of Bacillus thuringensis Isolates from Tropical Rain Forest Reserves of Hainan Island[J].Journal of Guangxi Agricultural and Biological Science,2009(2):265-274. |
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| Authors: | Zhang Wenfei Quan Jiaxin Xie Liu Wang Xi Yi Yantao Feng Meimei Zhu Lin Wang Ruiping Fang Xuanjun Haide Institute of Tropical Agricultural Resources Sanya College of Life Science Technology Guangxi University Nanning College of Life Sciences Hainan Normal University Haikou |
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| Institution: | Zhang Wenfei 1,2 Quan Jiaxin 2 Xie Liu 1,2 Wang Xi 1,2 Yi Yantao 1 Feng Meimei 1 Zhu Lin 3 Wang Ruiping 3 Fang Xuanjun 1 1 Haide Institute of Tropical Agricultural Resources,Sanya,572025,2 College of Life Science , Technology,Guangxi University,Nanning,530005,3 College of Life Sciences,Hainan Normal University,Haikou,571158 |
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| Abstract: | In present research we conducted the collection of soil samples from tropical rain forest reserves of Hainan island and isolation of Bacillus and identification of Bacillus thuringensis. Totally 1 882 soil samples were collected from the tropical rain forest reserves of Jianfenglin,Wuzhishan,Diaoloushan and Bawanglin,3 924 bacillus isolates were harvested based on the approach of selection of sodium acetate culture medium and treatment of high temperature,and 158 Bacillus isolates were further identified as... |
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| Keywords: | Bacillus Bacillus thuringensis Parasporal crystal protein cry genotypes Tropical rain forest reserves of Hainan Island |
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