| 无核葡萄与中国野生葡萄杂种的胚挽救技术研究 |
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| 引用本文： | 王飞,王跃进,周建锡.无核葡萄与中国野生葡萄杂种的胚挽救技术研究[J].园艺学报,2006,33(5):1079-1082. |
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| 作者姓名： | 王飞 王跃进 周建锡 |
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| 作者单位： | (西北农林科技大学园艺学院, 陕西杨凌712100) |
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| 基金项目： | 国家自然科学基金项目(30370993)，国家高技术研究发展计划(863计划)项目(2001AA241172) |
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| 摘 要： | 通过无核葡萄与中国野生葡萄杂交, 获得了4个杂交组合的后代植株, 确定了各杂交组合取胚珠培养的最佳时期。1Flame ×山葡萄; 2红宝石×爱莫无核; 3红宝石×北醇; 4爱莫无核×山葡萄在授粉后45 d取样培养效果较佳; 5长穗无核白×山葡萄授粉后30 d; 6无核白自交在授粉后35 d培养效果较佳。以NitSchs为基本培养基, 附加0.5 mg·L - 1 6-BA + 0.5 mg·L-1 GA3 + 2.5 mg·L - 1 IBA + 0.1 mg·L-1ZT, 适合于1、3、5号杂交组合; 而0.5 mg·L-1 6-BA + 0.5 mg·L-1 GA3 + 2.0 mg·L-1 IBA + 0.1 mg·L-1ZT适合2、4、6号杂交组合; 胚萌发培养基为2.0 mg·L-1 IBA + 1.0 mg·L-1 6-BA +0.2 mg·L-1 GA3 , 适合1、2、3、5、6号杂交组合, 而爱莫×山葡萄在1.5 mg·L - 1 IBA + 1.0 mg·L-1 6-BA + 0.2 mg·L-1 GA3培养基上表现较佳, 生根培养基为1 /2MS基本培养基添加0.15 mg·L-1 IBA + 0.02 mg·L-1 6-BA, 它适合1号与5号组合, 0.10 mg·L-1 IBA + 0.02 mg·L-1 6-BA适合4号与6号组合。
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| 关 键 词： | 无核葡萄 中国野生葡萄 杂种 胚挽救 |
| 文章编号： | 0513-353X（2006）05-1079-04 |
| 收稿时间： | 2006-01-25 |
| 修稿时间： | 2006-01-252006-05-18 |
Breeding Technology of Embryo Rescue in Seedless Grape with Chinese wild Grape |
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| Wang Fei,Wang Yuejin,Zhou Jianxi.Breeding Technology of Embryo Rescue in Seedless Grape with Chinese wild Grape[J].Acta Horticulturae Sinica,2006,33(5):1079-1082. |
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| Authors: | Wang Fei Wang Yuejin Zhou Jianxi |
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| Institution: | (Northwest Science and Technology University of Agriculture and Forestry, Yangling, Shaanxi 712100, China) |
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| Abstract: | Through crossing between seedless grapes and Chinese wild grapes we got four group hybrid grape seedlings. The best time to harvest the hybrid grapes ovule for embryo rescued were as follows: 45 d after pollination are (1) Flame seedless with Vitis amurensis, (2) Ruby seedless with Olmo seedless, (3)Ruby seedless with Beichun, (4) Olmo seedless with Vitis amurensis; 30 d after pollination is (5) Changsui saltanina seedless with Vitis amarensis; 35 d after pollination is (6) Self-pollinated saltanina. Nistschs medium with additional 0.5 mg·L - 1 6-BA + 0.5 mg·L-1 GA3 + 2.5 mg·L-1 IBA + 0.1 mg·L-1 ZT was suitable for cross (1) , (3) and (5) , but Nistschs medium with additional 0.5 mg·L-1 6-BA + 0.5 mg·L-1GA3 + 2.0 mg·L-1 IBA + 0.1 mg·L-1 ZT was suitable for cross (2) , (4) and (6). For embryo regeneration Nistschs medium with additional 2.0 mg·L-1 IBA + 1.0 mg·L-1 6-BA + GA3 0.2 mg·L-1 was suitable for cross (1) , (2) , (3) , (5) and (6) , but for cross (4) medium with 1.5 mg·L-1 IBA + 0.2 mg·L - 1 GA3 + 1.0 mg·L - 1 6-BA was more suitable. 1/2MS medium adding 0.15 mg·L - 1 IBA + 0.02 mg·L - 1 6-BA were suitable for cross (1) and (5) to acquire embryo rescue plants, but 1 /2MS medium with addition 0.1 mg·L - 1 IBA + 0.02 mg·L - 1 6-BA was suitable for cross (2) and (4). |
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| Keywords: | Seedless grape Chinese wild grape Hybrid Embryo rescue |
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