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| 泥炭土壤上与胞外酶相关的土壤微生物体与Marsh orchid的交互作用 | |
| 作者姓名： | H. KANG C. FREEMAN |
| 作者单位： | [1]Department of Civil and Environmental Engineering, Yonsei University, Seoul 120- 749 （Korea） [2]School of Biological Sciences, University of Wales, Bangor, LL57 2UW （UK） |
| 基金项目： | 1Project supported by the Advanced Environmental Biotechnology Research Center （AEBRC）, Korea; the Korea Science and Engineering Foundation; and the Ecotopia 21 of Ministry of Environment, Korea. |
| 摘 要： | The nature of the interactions between microbes and roots of plants in a peaty soil were studied in a laboratorybased experiment by measuring activities of β-glucosidase, phosphatase, N-acetylglucosaminidase, and arylsulphatase. The experiment was based on control （autoclaved）, bacteria-inoculated, and plant （transplanted with Dactylorhiza） treatments, and samples were collected over 4 sampling intervals. Higher enzyme activities were associated with the bacteria-inoculated treatment, suggesting that soil enzyme activities are mainly of microbial origin. For example,β-glucosidase activity varied between 25-30μmol g^-1 min^-1 in the bacteria-inoculated samples whilst the activity of the control ranged between 4-12 μmol g^-1 min^-1. A similar pattern was found for all other enzymes.
At the end of the incubation, the microcosms were destructively sampled and the enzyme activities determined in bulk soil, rhizospheric soil, and on the root surface. Detailed measurement in different fractions of the peat indicated that higher activities were found in rhizosphere. However, the higher activities of β-glucosidase, N-acetylglucosaminidase, and arylsulphatase appeared to be associated with bacterial proliferation on the root surface, whilst a larger proportion of phosphatase appeared to be released from root surface. |
| 关 键 词： | 湿地 根歧 细胞外酶 泥炭 |
| 收稿时间： | 2 June 2007 |
| 修稿时间： | 2007-06-02 |
Interactions of marsh orchid (Dactylorhiza spp.) and soil microorganisms in relation to extracellular enzyme activities in a peat soil |
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| H. KANG,C. FREEMAN.Interactions of marsh orchid (Dactylorhiza spp.) and soil microorganisms in relation to extracellular enzyme activities in a peat soil[J].Pedosphere,2007,17(6):681-687. | |
| Authors: | H KANG and C FREEMAN |
| Institution: | aDepartment of Civil and Environmental Engineering, Yonsei University, Seoul 120-749 (Korea) bSchool of Biological Sciences, University of Wales, Bangor, LL57 2UW (UK) |
| Abstract: | The nature of the interactions between microbes and roots of plants in a peaty soil were studied in a laboratory-based experiment by measuring activities of β-glucosidase, phosphatase, N-acetylglucosaminidase, and arylsulphatase. Theexperiment was based on control (autoclaved), bacteria-inoculated, and plant (transplanted with Dactylorhiza) treatments, and samples were collected over 4 sampling intervals. Higher enzyme activities were associated with the bacteria-inoculated treatment, suggesting that soil enzyme activities are mainly of microbial origin. For example, β-glucosidase activity varied between 25-30μmol g?1 min?1 in the bacteria-inoculated samples whilst the activity of the control ranged between 4-12μmol g?1 min?1. A similar pattern was found for all other enzymes. At the end of the incubation, the microcosms were destructively sampled and the enzyme activities determined in bulk soil, rhizospheric soil, and on the root surface. Detailed measurement in different fractions of the peat indicated that higher activities were found in rhizosphere. However, the higher activities of β-glucosidase, N-acetylglucosaminidase, and arylsulphatase appeared to be associated with bacterial proliferation on the root surface, whilst a larger proportion of phosphatase appeared to be released from root surface. |
| Keywords: | Dactylorhiza extracellular enzyme peat rhizosphere wetland |
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