Large-scale production of wheat and triticale double haploids through the use of a single-anther culture method |
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| Authors: | S Tuvesson A Ljungberg N Johansson † KE Karlsson L W Suijs JP Josset |
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| Institution: | Svalöf Weibull AB, SE-26881 Svalöv, Sweden |
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| Abstract: | A total of 257 parental wheat and 38 triticale lines were used for anther culture. On average, 2.1 green wheat haploids were obtained per spike. This response occurred irrespective of the origin of the material (Germany, France, Sweden or UK) and 5 years of testing. Triticale responded with 5.3 green haploids per spike. Using the criterion that one parental line should give at least one green haploid per spike in the screening experiment, green haploids were produced from 88 out of 91 F1 wheat breeding combinations and from each of 21 F1 and F2 triticale breeding combinations. An average of 4.7 green plants were obtained per spike from the wheat production programme, while the triticale programme gave an average of 6.2 green plants per spike. A single medium supplemented with different hormones for anthers and embryos was used for culture of both crops. |
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| Keywords: | Triticum aestivum X Triticosecale anther culture double haploid production plant regeneration |
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