| 仙客来体细胞胚发生和发育过程中淀粉粒的变化 |
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| 引用本文： | 于文胜,姜伟,龚雪琴,罗珍珍,解晓旭,由翠荣.仙客来体细胞胚发生和发育过程中淀粉粒的变化[J].园艺学报,2013,40(8):1527-1534. |
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| 作者姓名： | 于文胜 姜伟 龚雪琴 罗珍珍 解晓旭 由翠荣 |
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| 作者单位： | （1烟台市园林管理处，山东烟台 264000；2烟台大学生命科学学院，山东烟台 264005） |
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| 基金项目： | 烟台市科技发展计划项目(2011045);山东省科技发展计划项目(2007GG20002019) |
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| 摘 要： | 以仙客来（Cyclamen persicum Mill.）开花植株的新生叶片为外植体，诱导筛选胚性愈伤组织，并使其进一步发育成体细胞胚。组织切片观察结果表明：胚性愈伤组织由胚性和非胚性细胞组成，胚性细胞多以胚性细胞团的形式存在，胚性细胞团起源于诱导的胚性决定细胞。体细胞胚起源于单个胚性细胞，经多细胞原胚、球形胚、心形胚和鱼雷胚等时期发育成完整植株。在体细胞胚的发生发育过程中，淀粉粒出现4次积累高峰，分别为胚性细胞、球形胚、早期鱼雷胚和成熟胚发育成完整的小植株时期，淀粉代谢与体细胞胚发生、发育及小植株的形态建成密切相关。
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| 关 键 词： | 仙客来 体细胞胚 诱导的胚性决定细胞 淀粉粒 |
| 收稿时间： | 2013-04-12 |
Dynamic Changes of Starch Granules During Somatic Embryogenesis and Development in Cyclamen persicum |
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| YU Wen-sheng,JIANG Wei,GONG Xue-qin,LUO Zhen-zhen,XIE Xiao-xu,and YOU Cui-rong.Dynamic Changes of Starch Granules During Somatic Embryogenesis and Development in Cyclamen persicum[J].Acta Horticulturae Sinica,2013,40(8):1527-1534. |
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| Authors: | YU Wen-sheng JIANG Wei GONG Xue-qin LUO Zhen-zhen XIE Xiao-xu and YOU Cui-rong |
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| Institution: | （1Garden Management Department of Yantai，Yantai，Shandong 264000，China；2College of Life Science，Yantai University，Yantai，Shandong 264005，China） |
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| Abstract: | Embryogenic calluses were induced and filtrated from young leaf explants in flowering Cyclamen persicum plant，and somatic embryos were formed. Histological observation revealed that embryogenic calluses were comprised of embryogenic and non-embryogenic cells，and that embryogenic cells mostly existed in embryogenic aggregates. Embryogenic aggregates derived from induced embryogenic determined cells. Somatic embryogenesis originated from a single embryogenic cell. Somatic embryo grew into a complete plantlet after passing through proembryos，globular，heart- and torpedo- shaped stages. During somatic embryogenesis and development，four peaks of starch granules appeared，respectively at the stage of embryogenic cells，globular embryos，early torpedo-shaped embryos and complete plantlets from mature embryos. Starch metabolism was closely related to somatic embryogenesis，development and plantlet regeneration. |
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| Keywords: | Cyclamen persicum Cyclamen persicum somatic embryo somatic embryo induced embryogenic determined cell starch granule |
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