Distinct phylogenetic positions of <Emphasis Type="Italic">Grapevine fanleaf virus</Emphasis> isolates from Iran based on the movement protein gene |
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| Authors: | Nemat Sokhandan Bashir Shahrzad Nikkhah Mohammad Hajizadeh |
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| Institution: | (1) Plant Protection Department, University of Tabriz, Tabriz, 51664, Iran |
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| Abstract: | In DAS-ELISAs of 86 grapevine samples from northwestern Iran, Grapevine fanleaf virus (GFLV) was detected in 18 samples. RT-PCR with two primer pairs (M2/M4 or M0/M4) corresponding to GFLV movement protein (MP)
amplified the expected 854- and/or 1,489-bp fragment(s), respectively, from all ELISA-positive samples. Four smaller and three
larger PCR products were cloned and sequenced, which revealed that the MP region of the isolates was 1,044 nucleotides (nt)
long, corresponding to the GFLV MP. There were 83–86% nucleotide and 93–94% amino acid identities deduced between the MPs
of the sequenced isolates. Nucleotide sequence identities of 81–87 and 75–79% were found between the MP regions of these isolates
and that of previously published GFLV and Arabis mosaic virus (ArMV) strains/isolates, respectively. On a consensus parsimony tree based on the nucleotide sequences, isolates La208 and
X300 remained distinct from previously reported GFLVs. This is the first molecular characterization of GFLV MP isolates from
Iran.
The sequence data reported in this paper have been submitted to the DDBJ/EMBL/GenBank databases and have been assigned accession
numbers DQ286901 to DQ286916. |
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| Keywords: | ArMV Consensus parsimony DAS-ELISA GFLV Movement protein Phylogenetic analysis RT-PCR Sequence alignment |
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