| 宁夏地区玉米镰孢茎腐病病原菌的分离与鉴定 |
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| 引用本文： | 张小杰,王春明,周天旺,刘浩,沈瑞清,李敏权,郭成.宁夏地区玉米镰孢茎腐病病原菌的分离与鉴定[J].草地学报,2021,29(4):805-812. |
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| 作者姓名： | 张小杰 王春明 周天旺 刘浩 沈瑞清 李敏权 郭成 |
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| 作者单位： | 1.甘肃农业大学植物保护学院, 甘肃 兰州 730070;2.甘肃省农业科学院植物保护研究所, 甘肃 兰州 730070;3.宁夏农林科学院植物保护研究所, 宁夏 银川 750002 |
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| 基金项目： | 宁夏农林科学院对外科技合作专项(DW-X-2018019);宁夏回族自治区重点研发项目(2018BBF02018-15)资助。 |
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| 摘 要： | 为明确宁夏地区玉米镰孢茎腐病病原菌的种类及优势病原菌,本研究于2020年采集宁夏地区玉米(Zea mays L.)茎腐病样品36份,对所采集样品进行组织学分离、纯化和单孢分离,参照Nelson等分类系统进行形态学鉴定,并结合镰孢菌特异性引物EF-1α进行序列分析。本研究共获得209株镰孢菌,包括禾谷镰孢复合种(Fusarium graminearum species complex)、拟轮枝镰孢(F. verticillioides)、木贼镰孢(F. equiseti)、层出镰孢(F. proliferatum)、胶孢镰孢(F. subglutinans)、半裸镰孢(F. incarnatum)、锐顶镰孢(F.acuminatum.)、黄色镰孢(F.culmorum)和燕麦镰孢(F.avenaceum),其分离频率依次为28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9%和1.0%。其中,禾谷镰孢复合种在宁夏5市均有分布,且致病性强,为宁夏地区玉米镰孢茎腐病优势致病菌。
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| 关 键 词： | 宁夏 玉米茎腐病 镰孢菌 TEF序列鉴定 |
| 收稿时间： | 2020-12-01 |
Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia |
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| ZHANG Xiao-jie,WANG Chun-ming,ZHOU Tian-wang,LIU Hao,SHEN Rui-qing,LI Min-quan,GUO Cheng.Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia[J].Acta Agrestia Sinica,2021,29(4):805-812. |
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| Authors: | ZHANG Xiao-jie WANG Chun-ming ZHOU Tian-wang LIU Hao SHEN Rui-qing LI Min-quan GUO Cheng |
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| Institution: | 1. College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China;3 Plant Protection Institute, Ningxia Agriculture and Forestry Academy, Yinchuan, Ningxia 750002, China |
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| Abstract: | In order to clarify the species diversity and dominant species of Fusarium causing maize stalk rot in Ningxia,in 2020,36 samples of maize stalk rot in Ningxia were collected.After tissue isolation,purification,and single-spore isolation,the obtained isolates were morphologically identified following Nelson and other classification systems,and molecularly determined according to their EF-1α sequences.The results showed that a total of 209 strains of Fusarium were obtained,including Fusarium graminearum species complex,F.verticillioides,F.equiseti,F.proliferatum,F.subglutinans,F.incarnatum,F.acuminatum,F.culmorum and F.avenaceum,whose isolation frequencies were 28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9% and 1.0% respectively.Among them,F.graminearum species complex was distributed in all the five cities of Ningxia,and its pathogenicity was strong,so it was the dominant pathogen of maize stalk rot in Ningxia. |
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| Keywords: | Ningxia Maize Stalk Rot Fusarium TEF sequence identification |
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