| 苜蓿抗寒性与根系抗氧化酶活性相关性分析 |
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| 引用本文： | 张玉霞,丛百明,王显国,张庆昕,杜晓艳,田永雷.苜蓿抗寒性与根系抗氧化酶活性相关性分析[J].草地学报,2021,29(2):244-249. |
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| 作者姓名： | 张玉霞 丛百明 王显国 张庆昕 杜晓艳 田永雷 |
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| 作者单位： | 1. 内蒙古民族大学, 内蒙古 通辽 028041;2. 内蒙古通辽市农牧业局, 内蒙古 通辽 028000;3. 中国农业大学草业科学与技术学院, 北京 100083;4. 内蒙古自治区农牧科学院, 内蒙古 呼和浩特 010013 |
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| 基金项目： | 内蒙古自治区科技储备项目;国家公益性行业(农业)公益项目;内蒙古自然科学基金项目 |
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| 摘 要： | 为探究不同苜蓿材料的抗寒性与低温胁迫下苜蓿根颈抗氧化酶活性变化的关系，本研究在科尔沁沙地种植黄花苜蓿（Medicago falcata L.）和4个紫花苜蓿（Medicago sativa L.）品种，越冬前挖取越冬器官并进行-10℃，-15℃，-20℃，-25℃，-30℃低温胁迫处理，以低温（4℃）贮藏为对照，测定苜蓿根颈抗氧化酶活性及相对电导率，利用相对电导率值拟合Logistic回归方程计算苜蓿半数致死温度（LT50），进行LT50与抗氧化酶活性相关性分析。结果表明，黄花苜蓿的抗寒性强于紫花苜蓿，其中紫花苜蓿‘北极熊’品种抗寒性最强，‘骑士T’品种最弱。LT50与不同温度处理下抗氧化酶活性呈负相关，在-30℃处理下超氧化物歧化酶（Superoxide dismutase，SOD）活性和过氧化氢酶（Catalase，CAT）活性分别与LT50呈显著和极显著负相关。综上所述，苜蓿通过提高SOD，CAT活性增强抗寒性，-30℃处理下的SOD活性和-10℃，-30℃处理下CAT活性可以作为衡量苜蓿抗寒性强弱的生理指标。
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| 关 键 词： | 低温胁迫 苜蓿 抗氧化酶 抗寒性 |
| 收稿时间： | 2020-09-23 |
Correlation Analysis of Cold Resistance and Antioxidant Enzyme Activities in Alfalfa Roots |
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| ZHANG Yu-xia,CONG Bai-ming,WANG Xian-guo,ZHANG Qing-xin,DU Xiao-yan,TIAN Yong-lei.Correlation Analysis of Cold Resistance and Antioxidant Enzyme Activities in Alfalfa Roots[J].Acta Agrestia Sinica,2021,29(2):244-249. |
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| Authors: | ZHANG Yu-xia CONG Bai-ming WANG Xian-guo ZHANG Qing-xin DU Xiao-yan TIAN Yong-lei |
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| Institution: | 1. Inner Mongolia University for the Nationalities, Tongliao 028041, Inner Mongolia, China;2. Agriculture and Animal Husbandry Bureau of Tongliao City, Tongliao, Inner Mongolia 028000, China;3. College of Grassland Science and Technology, China Agricultural University, Beijing 100083, China;4. College of Agriculture and Animal Husbandry, Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia 010013, China |
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| Abstract: | In order to explore the relationship between the cold resistance of different alfalfa materials and the changes of antioxidant enzyme activity in alfalfa root crown under low temperature stress,sickle alfalfa (Medicago falcata L.) and four alfalfa (Medicago sativa L.) varieties were planted in Horqin sandy land. Overwintering organs were harvested before winter,and were treated with -10℃,-15℃,-20℃,-25℃ and -30℃. The antioxidant enzyme activity and relative conductivity of alfalfa root neck were measured with low temperature (4℃) storage as control (CK). Fitting logistic regression equation with relative conductivity to calculate semi-lethal temperature (LT50) of alfalfa. The correlation between LT50 and antioxidant enzyme activity was analyzed. The results showed that the cold resistance of sickle alfalfa was stronger than that of Medicago sativa. Among Medicago sativa,‘polar bear’ had the strongest cold resistance while ‘Knight T’ had the weakest. LT50 was negatively correlated with antioxidant enzyme activities at different temperatures. The uperoxide dismutase (SOD) and Catalase (CAT) activities in alfalfa root crown under -30℃ treatment had significant and extremely significant negative correlation with LT50. Conclusion:alfalfa enhanced cold resistance by increasing SOD and CAT activities. SOD activity at -30℃ and CAT activities at -10℃ and -30℃ can be used as physiological indexes of alfalfa cold resistance. |
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| Keywords: | Low temperature stress Alfalfa Antioxidant enzymes Cold resistance |
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