Effects of Perioperative Granulocyte Colony-Stimulating Factor on Horses With Ascending Colonic Ischemia |
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| Authors: | KENT E SULLIVAN vmd JACK R SNYDER dvm PhD Dipiomate ACVS JOHN E MADIGAN DVM MS Dipiomate ACVIM JOHN R PASCOE BVSc PhD Dipiomate ACVS THOMAS B FARVER PhD MARK C THURMOND dvm PhD JEFF W ANDRESEN PhD |
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| Institution: | Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis;Departments of Surgery, School of Veterinary Medicine, University of California, Davis, and Amgen Inc;Departments of Medicine, School of Veterinary Medicine, University of California, Davis;Departments of Epidemiology &Preventive Medicine, School of Veterinary Medicine, University of California, Davis;Amgen Inc., Thousand Oaks, California. |
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| Abstract: | Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein that regulates the proliferation and maturation of hematopoietic progenitor cells and modulates the function of mature neu-trophils. The responses to administration of G-CSF alone, and in combination with antimicrobials, were studied in an equine model of ascending colon ischemia. Complete segmental colonic ischemia (3.75 hours) with pelvic flexure enterotomy was created in four treatment groups. Group 1 horses received recombinant canine G-CSF (10 μg/kg, every 24 hours, intramuscularly), gentamicin sulfate (2.2 mg/kg, every 8 hours, intravenously), and potassium penicillin G (40,000 lU/kg, every 6 hours, intravenously). Group 2 horses were treated with the G-CSF vehicle and antimicrobials as for group 1. Group 3 horses received G-CSF and the antimicrobial drug vehicles, and group 4 horses served as the untreated control receiving G-CSF vehicle and antimicrobial vehicles. The results for 20 horses, five horses in each group, were compared. Treatment with G-CSF was associated with an increased concentration of white blood cells, band neutrophils, neutrophils, lymphocytes, and monocytes in the peripheral blood after surgery. Antimicrobial administration had no detectable effect on cell concentrations after surgery. Administration of G-CSF was associated with an increased concentration of nucleated cells in the peritoneal fluid including neutrophils, small mononuclear cells and large mononuclear cells. Horses that developed incisional infections had lower neutrophil concentrations in the peripheral blood on postoperative day 2 than horses without infected incisions. These results suggested that the prophylactic administration of G-CSF may be useful in the treatment of patients at risk for developing neutropenia after surgery. |
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