| 棉花枯萎病菌生理小种的分子指纹分析 |
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| 引用本文： | 冯洁,孙文姬,石磊岩,马存.棉花枯萎病菌生理小种的分子指纹分析[J].棉花学报,1999(5). |
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| 作者姓名： | 冯洁 孙文姬 石磊岩 马存 |
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| 作者单位： | 中国农业科学院植物保护研究所!北京100094 |
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| 基金项目： | 植物病虫害生物学国家重点实验室开放基金 |
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| 摘 要： | 以我国棉花枯萎菌３个生理小种的２６个代表菌株及国外３ 个不同生理小种菌株进行随机扩增多态性ＤＮＡ（ＲＡＰＤ）分析，共产生了１４０个ＲＡＰＤ分子标记，其中８７８％ 具有多态性。通过聚类分析将供试菌株划分为６个ＲＡＰＤ组，确定了不同小种间的亲缘关系，为确立我国棉花枯萎菌生理小种在国际上的分类地位提供了可靠的分子证据。
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| 关 键 词： | 棉花 枯萎菌 生理小种 RAPD分析 |
RAPD Analysis of Physiologic Races of Fusarium oxysporum f. sp. vasinfectum in China |
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| FENG jie,SUN Wen ji,SHI Lei yan,MA Cun.RAPD Analysis of Physiologic Races of Fusarium oxysporum f. sp. vasinfectum in China[J].Cotton Science,1999(5). |
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| Authors: | FENG jie SUN Wen ji SHI Lei yan MA Cun |
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| Abstract: | Random amplified polymorphic DNA(RAPD) markers was used to assess genetic diversity among 3 races of Fusarium oxysporum f.sp. vasinfectum in China and 3 isolates from foreign origin were amplified with 10 decamer primers, 140 RAPD markers were obtained, 87 8% were polymorphisms. Genetic relationships among the isolates were determined by hierarchical cluster method. RAPD analysis can provide reliable molecular evidences of determine classification position of Fusarium oxysporum f.sp. vasinfectum of China in the world. |
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| Keywords: | cotton Fusarium wilt physiologic race RAPD analysis |
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