| Analysis of SSR Fingerprints in Introduced Silkworm Germplasm Resources |
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| 作者姓名： | HOU Cheng-xiang LI Mu-wang ZHANG Yue-hua QIAN He-ying SUN Ping-jiang XU An-ying MIAO Xue-xia GUO Qiu-hong XIANG Hui HUANG Yong-ping |
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| 作者单位： | [1]Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, P.R.China [2]Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, P.R. China |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Thirty-five SSR markers were used to construct 96 silkworm races fingerprint. All the SSR markers were polymorphic and unambiguously separated silkworm strains from each other. A total of 467 alleles were detected with a mean value of 13.34 alleles/locus （range 3-28）. The mean polymorphism index content （PIC） was 0.71 （range 0.299-0.919）. UPGMA cluster analysis of Nei＇s genetic distance grouped silkworm strains on the basis of their origin. The results indicated that SSR markers are efficient tools for fingerprinting cultivars and conducting genetic diversity studies in the silkworm.
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| 关 键 词： | 家蚕 引进种质资源 SSR指纹 遗传分析 |
Analysis of SSR Fingerprints in Introduced Silkworm Germplasm Resources |
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| HOU Cheng-xiang LI Mu-wang ZHANG Yue-hua QIAN He-ying SUN Ping-jiang XU An-ying MIAO Xue-xia GUO Qiu-hong XIANG Hui HUANG Yong-ping.Analysis of SSR Fingerprints in Introduced Silkworm Germplasm Resources[J].Agricultural Sciences in China,2007,6(5):620-627. |
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| Authors: | HOU Cheng-xiang LI Mu-wang ZHANG Yue-hua QIAN He-ying SUN Ping-jiang XU An-ying MIAO Xue-xia GUO Qiu-hong XIANG Hui HUANG Yong-ping |
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| Institution: | 1. College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China;2. Institute of Sericulture, Chengde Medical University, Chengde 067000, PR China |
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| Abstract: | Thirty-five SSR markers were used to construct 96 silkworm races fingerprint. All the SSR markers were polymorphic and unambiguously separated silkworm strains from each other. A total of 467 alleles were detected with a mean value of 13.34 alleles/locus (range 3-28). The mean polymorphism index content (PIC) was 0.71 (range 0.299-0.919). UPGMA cluster analysis of Nei's genetic distance grouped silkworm strains on the basis of their origin. The results indicated that SSR markers are efficient tools for fingerprinting cultivars and conducting genetic diversity studies in the silkworm. |
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| Keywords: | silkworm (Bombyx mori L ) germplasm SSR marker fingerprint |
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