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| 兔出血症病毒VP60蛋白单克隆抗体的制备及鉴定 | |
| 引用本文： | 魏威,余斌,倪征,陈柳,云涛,华炯钢,吴润,刘光清.兔出血症病毒VP60蛋白单克隆抗体的制备及鉴定[J].甘肃农业大学学报,2009,44(1). |
| 作者姓名： | 魏威 余斌 倪征 陈柳 云涛 华炯钢 吴润 刘光清 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;浙江省农业科学院农业部病毒学与生物技术重点实验室,浙江,杭州,310021 2. 浙江省农业科学院农业部病毒学与生物技术重点实验室,浙江,杭州,310021 3. 甘肃农业大学动物医学院,甘肃,兰州,730070 |
| 基金项目： | 国家自然科学基金，浙江省自然科学基金 |
| 摘 要： | 以纯化的重组兔出血症病毒核衣壳蛋白(VP60)为抗原,免疫BALB/c小鼠,采用杂交瘤细胞技术制备抗衣壳蛋白的单克隆抗体,获得了2株能稳定分泌抗VP60蛋白单克隆抗体的杂交瘤细胞株(AE11和AH4).分别利用Western blot、间接ELISA和间接免疫荧光(IFA)等方法对单抗的免疫活性和特异性进行检测,结果证明,此两株单抗均能识别重组的VP60和天然病毒的衣壳蛋白,而IFA结果显示只有AE11能和RHDV反应,可观察到强烈的特异性绿色荧光,表明成功制备了抗RHDV的VP60特异性单抗. |
| 关 键 词： | 兔出血症病毒 衣壳蛋白 单克隆抗体 |
Preparation and identification of monoclonal antibodies against VP60 of RHDV |
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| WEI Wei,YU Bin,NI Zheng,CHEN Liu,YUN Tao,HUA Jiong-gang,WU Run,LIU Guang-qing.Preparation and identification of monoclonal antibodies against VP60 of RHDV[J].Journal of Gansu Agricultural University,2009,44(1). | |
| Authors: | WEI Wei YU Bin NI Zheng CHEN Liu YUN Tao HUA Jiong-gang WU Run LIU Guang-qing |
| Institution: | WEI Wei1,2,YU Bin2,NI Zheng2,CHEN Liu2,YUN Tao2,HUA Jiong-gang2,WU Run1,LIU Guang-qing2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.Key Laboratory of Virology and Biotechnology of Agriculture Ministry,Zhejiang Academy of Agriculture Sciences,Hangzhou 310021,China) |
| Abstract: | BALB/c mice were immunized with the recombinated capsid protein(VP60) of Rabit hemorrhagic disease virus(RHDV),and anti-VP60 mAb was prepared by using hybridoma techonology.The specificity of mAbs(AE11 and AH4) was characterized by Western-blot and indirect enzyme linked immunosorbent assay(ELISA).The results indicated that the mAbs was able to recognize the recombinated protein(rVP60) and the natural viral protein.However,the result of IFA indicated that only AE11 could react to RHDV,and the infected cells... |
| Keywords: | rabit hemorrhagic disease virus capsid protein monoclonal antibody |
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