A significant proportion of indigenous rhizobia from India associated with soybean (<Emphasis Type="Italic">Glycine max</Emphasis> L.) distinctly belong to <Emphasis Type="Italic">Bradyrhizobium</Emphasis> and <Emphasis Type="Italic">Ensifer</Emphasis> genera |
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| Authors: | Chinnaswamy Appunu Narayanasamy Sasirekha Vaiyapuri Ramalingam Prabavathy Sudha Nair |
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| Institution: | (1) Division of Microbiology, M. S. Swaminathan Research Foundation, III Cross Street, Taramani Institutional Area, 600113 Chennai, Tamil Nadu, India;(2) Present address: Division of Crop Improvement, Sugarcane Breeding Institute, Coimbatore, 641 007, Tamil Nadu, India |
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| Abstract: | The diversity among 269 rhizobia isolated from naturally occurring root nodules of soybean collected from two different agro-ecological
regions of India, based on RFLP and sequences of the intergenic spacer (IGS) between the 16S and 23S rRNA genes, growth rate,
and indole acetic acid production, revealed their significant, site-dependent genomic diversity. Among these bacteria, nine
IGS genotypes were identified with two endonucleases. They were distributed into five divergent lineages by sequence analysis
of each IGS representative strain, i.e., (1) comprising IGS genotypes I, II, III, and reference Bradyrhizobium yuanmingense; (2) with genotype IV and strains of unclassified bradyrhizobia genomic species; (3) including genotypes V, VI, and Bradyrhizobium liaoningense; (4) with IGS genotype VII and Bradyrhizobium elkanii strains; and (5) comprising IGS genotypes VIII, IX, and different Ensifer genus bacteria. Host-specificity test revealed that all rhizobia-nodulated soybean and cowpea and only part of them formed
nodules on Arachis hypogeae and Cajanus cajan. The great diversity of soybean nodulators observed in this study emphasises that Indian soil is an important reservoir of
nitrogen-fixing rhizobia. |
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