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| 肠炎沙门菌SEF14菌毛的抽提、纯化及生物学活性检测 | |
| 引用本文： | 陆广富,朱春红,段小丽,朱国强.肠炎沙门菌SEF14菌毛的抽提、纯化及生物学活性检测[J].中国家禽,2011,33(7). |
| 作者姓名： | 陆广富 朱春红 段小丽 朱国强 |
| 作者单位： | 1. 扬州大学兽医学院,江苏扬州,225009;江苏畜牧兽医职业技术学院,江苏泰州,225300 2. 江苏省家禽科学研究所,江苏扬州,225003 3. 扬州大学兽医学院,江苏扬州,225009 |
| 基金项目： | 国家自然科学基金，江苏省属高校自然科学重大基础研究项目 |
| 摘 要： | 试验从肠炎沙门菌国内标准株50336中抽提和纯化SEF14菌毛,并制备SEF14蛋白鼠源高免血清.结果发现,肠炎沙门菌在CFA培养液中,静止培养50 h左右,SEF14菌毛表达量相对较多,在机械高速匀浆后,经进一步的透析纯化,得到较纯的SEF14菌毛蛋白,蛋白大小和相关报道一致,约14 ku左右.Westem-blotting检测发现纯化的重组蛋白rSEFA(体外表达的SEF14菌毛主要亚单位蛋白SEFA)免疫小鼠得到的高免血清能识别标准株肠炎沙门菌SEF14菌毛蛋白以及纯化的重组蛋白rSEFA,说明体外抽提的SEF14菌毛蛋白和体外表达的rSEFA蛋白同样具有较好的免疫原性和反应原性. |
| 关 键 词： | 肠炎沙门菌 SEF14菌毛 抽提 纯化 |
Extraction, Purification and Immune Activity Analysis of SEF14 Fimbriae of S.enteritidis |
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| LU Guangfu,ZHU Chunhong,DUAN Xiaoli,ZHU Guoqiang.Extraction, Purification and Immune Activity Analysis of SEF14 Fimbriae of S.enteritidis[J].China Poultry,2011,33(7). | |
| Authors: | LU Guangfu ZHU Chunhong DUAN Xiaoli ZHU Guoqiang |
| Institution: | LU Guangfu1,2,ZHU Chunhong3,DUAN Xiaoli1,ZHU Guoqiang1** (1.College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,2.Jiangsu Animal Husbandry & Veterinary College,Taizhou,Jiangsu 225300,3.Jiangsu Institute of Poultry Science,Jiangsu 225003) |
| Abstract: | SEF14 fimbriae were extracted and purificated from S.enteritidis reference strain 50336,and high titer of anti-SEF14 mouse sera was made in this. The results showed that S.enteritidis could express SEF14 fimbriae by caltured in CFA medium statically in vitro. And SEF14 fimbriae was separated from S.enteritidis at room temperature by shearing and dialyzing,which of molecular weight was about 14 ku. High titer mouse sera of anti-rSEFA (recombinant subunit protein SEFA expressed in vitro) was prepared and dete... |
| Keywords: | S enteritidis SEF14 fimbriae extraction purification |
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