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硝酸钙处理对菊花扦插生根及抗氧化酶活性的影响
引用本文:郑成淑,王文莉,孙宪芝,梁芳,张翠华.硝酸钙处理对菊花扦插生根及抗氧化酶活性的影响[J].园艺学报,2008,35(2):263-268.
作者姓名:郑成淑  王文莉  孙宪芝  梁芳  张翠华
作者单位:(山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018)
基金项目:国家科技支撑计划项目 , 山东农业大学青年科技创新项目
摘    要: 以切花菊品种‘万盛’为材料,研究了不同浓度Ca(NO3)2处理对菊花扦插生根及其过程中插穗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性以及膜质过氧化产物丙二醛(MDA)含量的影响。结果表明,60 mg·L-1 Ca2+处理的生根数最多,而且插穗鲜样质量和干样质量也比对照明显增加,而80 mg·L-1Ca2+处理的生根数、插穗鲜样质量和干样质量以及生根率比对照减少。60 mg·L-1 Ca2+处理的SOD、POD、CAT和APX活性与其它处理相比显著增加,MDA含量明显减少。以上结果表明,在菊花扦插过程中,采用适当浓度的Ca2+处理可以提高插穗叶片SOD、POD、CAT和APX等保护酶的活性和降低MDA含量,从而促进菊花扦插生根,并提高扦插苗品质。

关 键 词:菊花    扦插  生根  抗氧化酶  丙二醛
文章编号:0513-353X(2008)02-0263-06
收稿时间:2007-07-18
修稿时间:2007-11-22

Effects of Ca2+ on Antioxidant Enzyme Activities During Rooting of Chrysanthemum Cuttings
ZHENG Cheng-shu,WANG Wen-li,SUN Xian-zhi,LIANG Fang,ZHANG Cui-hua.Effects of Ca2+ on Antioxidant Enzyme Activities During Rooting of Chrysanthemum Cuttings[J].Acta Horticulturae Sinica,2008,35(2):263-268.
Authors:ZHENG Cheng-shu  WANG Wen-li  SUN Xian-zhi  LIANG Fang  ZHANG Cui-hua
Institution:(College of Science and Technology, Shandong Agricultural University, State Key Laboratory of Crop Biology,Tai'an, Shandong 271018, China)
Abstract:Effects of Ca(NO3)2 under different concentrations on rooting and activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and ascorbate peroxidase (APX), and malondialdehyde (MDA) content in the leaves of cuttings of Dendrandema grandiflora 'Wangsheng' were studied. The results showed that the root number was the highest in 60 mg·L-1 Ca2+ treated cuttings with significant increase in fresh and dry weights of the cuttings compared to the controls. But root number, fresh and dry mass and rooting rates decreased at 80 mg·L-1 Ca2+. In 60 mg·L-1 Ca2+ treated plants, the activities of SOD, POD, CAT and APX increased and MDA content decreased significantly compared with other plants. These results indicated that Ca2+ treatments at proper dosage might enhance rooting of cuttings by increasing the activities of antioxidant enzymes and decreasing membrane lipid peroxidation during rooting in the cuttings of D. grandiflora.
Keywords:Chrysanthemum  2+  Rooting  Antioxidase  MDA
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