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含水量和添加剂对黄梁木叶青贮发酵品质的影响
引用本文:郭香,陈德奎,陈娜,李云,陈晓阳,张庆.含水量和添加剂对黄梁木叶青贮发酵品质的影响[J].草业学报,2021,30(8):199-205.
作者姓名:郭香  陈德奎  陈娜  李云  陈晓阳  张庆
作者单位:1.华南农业大学林学与风景园林学院,广东木本饲料工程技术研究中心,广东省森林植物种质创新与利用重点实验室,广东 广州 510642;2.华南农业大学动物科学学院,广东 广州 510642;3.北京林业大学生物科学与技术学院,北京 100083
基金项目:广东省林业科技创新项目(2018KJCX001);广东省自然基金项目(2020A1515011253)
摘    要:为了研究含水量和添加剂对黄梁木叶青贮发酵品质的影响,将两种不同含水量60%、75%的黄梁木叶片切碎后进行不加添加剂、添加6.00 log10 cfu·g-1植物乳杆菌(LP)、120 U·g-1纤维素酶(C)、6.00 log10 cfu·g-1植物乳杆菌和120 U·g-1纤维素酶(LP+C)处理,每个处理6组重复,装入塑料袋中,室温(约30 ℃)贮藏60 d后开袋。结果表明,黄梁木干物质中粗蛋白(CP)含量为12.48%,水溶性碳水化合物(WSC)含量为4.49%。75%含水量导致黄梁木叶青贮的pH值,乳酸菌(LAB)和酵母菌数量,中性洗涤纤维(NDF)含量,单宁含量,氨态氮(NH3-N)含量均显著降低(P<0.05),乳酸(LA)和乙酸(AA)含量显著升高(P<0.05)。LP和LP+C显著降低pH值、乳酸菌数和缩合单宁(CT)含量(P<0.05),显著提高乳酸含量(P<0.05)。同时,C和LP+C显著降低了中性洗涤纤维和酸性洗涤纤维(ADF)含量(P<0.05),显著提高了粗蛋白含量(P<0.05)。75%含水量且添加LP和LP+C的缩合单宁含量显著降低(P<0.05)。综上所述,75%含水量处理对黄梁木叶青贮发酵品质有积极的影响,使用添加剂可以改善青贮品质,以LP和C配合使用效果较好。

关 键 词:黄梁木叶  含水量  添加剂  青贮  发酵品质  
收稿时间:2020-06-23
修稿时间:2020-09-24

Effect of moisture content and additives on the fermentation quality of Neolamarckia cadamba leaf silage
GUO Xiang,CHEN De-kui,CHEN Na,LI Yun,CHEN Xiao-yang,ZHANG Qing.Effect of moisture content and additives on the fermentation quality of Neolamarckia cadamba leaf silage[J].Acta Prataculturae Sinica,2021,30(8):199-205.
Authors:GUO Xiang  CHEN De-kui  CHEN Na  LI Yun  CHEN Xiao-yang  ZHANG Qing
Institution:1.College of Forestry and Landscape Architecture,South China Agricultural University,Guangdong Research Center of Woody Forage Engineering Technology,Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,Guangzhou 510642,China;2.College of Animal Science,South China Agricultural University,Guangzhou 510642,China;3.College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China
Abstract:In order to study the effects of moisture content and additives on the fermentation quality of Neolamarckia cadamba leaf silage, two batches of N. cadamba leaves with different water contents of 60% and 75% were chopped and ensiled without additive, or with one of three additive treatments: inoculant containing 6.00 log10 cfu·g-1Lactobacillus plantarum (LP), 120 U·g-1 cellulase (C), or 6.00 log10 cfu·g-1 LP and 120 U·g-1 cellulase (LP+C). Six replicates of each treatment were weighed into plastic bags and stored at ambient temperature (about 30 ℃) for 60 days. Before ensiling the contents of crude protein and water-soluble carbohydrate in leaf dry matter were 12.48% and 4.49%, respectively. Compared to 60% water content, 75% water content caused a significant decrease in pH value, numbers of lactic acid bacteria and yeast cells, content of neutral detergent fiber, tannin content and ammonia nitrogen content of the silage (P<0.05), while the contents of lactic acid and acetic acid increased significantly (P<0.05). Treatments LP and LP+C significantly decreased the pH value, the number of lactic acid bacteria and the content of condensed tannins (P<0.05), while increasing the content of lactic acid (P<0.05). At the same time, C and LP+C significantly decreased the neutral and acid detergent fiber contents (P<0.05), and increased crude protein content (P<0.05). For the treatment with 75% water content and the additions of LP and LP+C, content of condensed tannins was were significantly reduced (P<0.05). In conclusion, the 75% water content treatment had a positive effect on the fermentation quality of N. cadamba leaf silage. Silage quality was improved by additives, and the combined application of LP and C gave optimal results.
Keywords:Neolamarckia cadamba leaves  moisture  additive  silage  fermentation quality  
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