| 锰胁迫下鸡眼草的富集特征及生理响应 |
| |
| 引用本文： | 李欣航,肖泽华,匡雪韶,王悟敏,罗亮宇,刘文胜.锰胁迫下鸡眼草的富集特征及生理响应[J].草业学报,2021,30(7):139-147. |
| |
| 作者姓名： | 李欣航 肖泽华 匡雪韶 王悟敏 罗亮宇 刘文胜 |
| |
| 作者单位： | 中南林业科技大学生命科学与技术学院，湖南 长沙 410004 |
| |
| 基金项目： | 湖南省教育厅项目(20C1917);湖南省研究生科技创新项目(CX2017B415) |
| |
| 摘 要： | 为探明植物对锰胁迫的耐受性机制，以采自重金属污染区与非污染区的鸡眼草为试验材料，在不同锰浓度胁迫下［0 （对照）、1000、5000、10000、15000、20000 μmol·L-1］ 开展盆栽试验，比较研究锰胁迫对两种来源鸡眼草表型、生理生化特性及锰积累特征的影响。结果表明：随着锰浓度的升高，1） 两种来源鸡眼草的根干重、芽干重、根冠比均呈降低的趋势；当锰浓度达5000~20000 μmol·L-1时，与对照相比，污染区、非污染区鸡眼草的芽干重分别下降4.34%~27.71%与16.33%~49.77%，根干重分别下降19.00%~66.06%与27.90%~77.54%，污染区下降幅度均较非污染区的小；2） 两种来源鸡眼草的叶绿素、可溶性糖、脯氨酸含量及超氧化物歧化酶（SOD）、过氧化物酶（POD）活性均呈先升后降的趋势，可溶性蛋白含量逐渐下降，丙二醛（MDA）含量则逐渐升高；3） 两种来源鸡眼草根、茎、叶的锰含量均增加；锰浓度为20000 μmol·L-1时，污染区和非污染区鸡眼草的叶锰含量分别为对照的16.53和13.41倍。因此，污染区鸡眼草锰耐受能力及富集能力均较非污染区鸡眼草高，较高的抗氧化酶活性是其耐受高锰胁迫的重要生理机制。
|
| 关 键 词： | 锰胁迫 重金属污染区 鸡眼草 生理生化 富集特征 |
| 收稿时间： | 2020-06-02 |
| 修稿时间： | 2020-08-19 |
Metal accumulation characteristics and physiological response of Kummerowia striata to manganese stress |
| |
| LI Xin-hang,XIAO Ze-hua,KUANG Xue-shao,WANG Wu-min,LUO Liang-yu,LIU Wen-sheng.Metal accumulation characteristics and physiological response of Kummerowia striata to manganese stress[J].Acta Prataculturae Sinica,2021,30(7):139-147. |
| |
| Authors: | LI Xin-hang XIAO Ze-hua KUANG Xue-shao WANG Wu-min LUO Liang-yu LIU Wen-sheng |
| |
| Institution: | College of Life Science and Technology，Central South University of Forestry and Technology，Changsha 410004，China |
| |
| Abstract: | This research studied the tolerance mechanism of plants to manganese stress. Individual plants of Kummerowia striata were collected from localities contaminated or not contaminated by heavy metals and cultivated under different concentrations of manganese ［0 （Control）， 1000， 5000， 10000， 15000， 20000 μmol·L-1］. The effects of manganese stress on the phenotype， physiological and biochemical features， and manganese accumulation characteristics of K. striata from these two sources were evaluated. It was found that， as the concentration of Mn increased： 1） The root dry weight， shoot dry weight， and root∶shoot ratio of K. striata from both localities decreased； when the concentration of Mn was in the range 5000-20000 μmol·L-1， compared with the Control， the shoot and root dry weight of plants from the contaminated locality decreased by 4.34%-27.71% and 19.00%-66.06%， respectively； while the shoot and root dry weight for plants from the uncontaminated locality decreased by 16.33%-49.77% and 27.90%-77.54%， respectively. 2） The contents of chlorophyll， soluble sugar and proline and the activities of superoxide dismutase and peroxidase in leaves of K. striata from the two localities showed a pattern of initial increase and then decrease with increasing Mn exposure， while the content of soluble protein gradually decreased and the content of malondialdehyde gradually increased. 3） The Mn content of the roots， stems， and leaves of K. striata from both localities increased. When grown at the Mn concentration of 20000 μmol·L-1， the Mn content of K. striata sourced from the contaminated and uncontaminated areas was 16.53 and 13.41 times than that of the Control， respectively. Therefore， the Mn accumulation capacity and Mn tolerance of the K. striata from the contaminated locality are higher than in plants sourced from the uncontaminated locality. High activities of antioxidant enzymes appear to be an important physiological mechanism of K. striata tolerance to high Mn stress. |
| |
| Keywords: | Mn stress heavy metal contaminated area Kummerowia striata physiological and biochemical features accumulation characteristics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《草业学报》浏览原始摘要信息 |
| 点击此处可从《草业学报》下载免费的PDF全文 |
