| 绵羊SPP1和PLCB3基因多态性与产羔数的关联分析 |
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| 引用本文： | 刁婉莹,狄冉,储明星.绵羊SPP1和PLCB3基因多态性与产羔数的关联分析[J].中国草食动物科学,2021(1):8-12. |
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| 作者姓名： | 刁婉莹 狄冉 储明星 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所 |
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| 基金项目： | 国家自然科学基金项目(31772580);国家肉羊产业技术体系专项(CARS-38);中国农业科学院科技创新工程(ASTIP-IAS13)。 |
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| 摘 要： | 试验旨在探究SPP1基因g.36651870T>C位点多态性、PLCB3基因g.41871219T>C位点多态性与绵羊产羔数之间的关系,以期寻找绵羊产羔数性状相关的分子标记.利用全基因组重测序结合Sequenom MassARRAY?SNP技术对多羔羊(小尾寒羊、湖羊、策勒黑羊)和单羔羊(滩羊、苏尼特羊、萨福克羊和草原...
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| 关 键 词： | 绵羊 SPP1基因 PLCB3基因 产羔数 SNP分型 |
Polymorphisms of SPP1 and PLCB3 Genes and Their Association with Litter Size in Sheep |
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| DIAO Wanying,DI Ran,CHU Mingxing.Polymorphisms of SPP1 and PLCB3 Genes and Their Association with Litter Size in Sheep[J].China Herbivores,2021(1):8-12. |
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| Authors: | DIAO Wanying DI Ran CHU Mingxing |
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| Institution: | (Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | This study aimed to explore the association of polymorphisms of SPP1 gene(g.36651870 T>C)and PLCB3 gene(g.41871219 T>C)and litter size in sheep,searching for new molecular markers for litter size trait in sheep.Based on whole-genome resequencing,Sequenom Mass ARRAY誖SNP technology was used to detect polymorphisms at g.36651870 T>C locus of SPP1 gene and g.41871219 T>C locus of PLCB3 gene in seven sheep breeds including polytocous sheep(Small-tail Han sheep,Hu sheep and Cele Black sheep)and monotocous sheep(Tan sheep,Sunite sheep,Suffolk sheep and Tibetan sheep),then the association between the loci and litter size in Small-tail Han sheep was analyzed.The results showed that TT,TC and CC genotypes were found at both loci.The genotype frequency and allele frequency of g.36651870 T>C locus were extremely significantly different between polytocous and monotocous sheep(P<0.01),and the allele frequency of g.41871219 T>C locus was significantly different between polytocous and monotocous sheep(P<0.05).Population genetic analysis indicated that the g.36651870 T>C locus was at moderate polymorphism in Small-tail Han sheep,Sunite sheep,Suffolk sheep and Hu sheep(0.25≤PIC<0.5),it was at low polymorphism in Tan sheep,Cele Black sheep and Tibetan sheep(PIC<0.25).The g.41871219 T>C locus was at moderate polymorphism in Tan sheep(0.25≤PIC<0.5),it was at low polymorphism in other six sheep breeds(PIC<0.25).Theχ2 test indicated that the g.36651870 T>C locus was under Hardy-Weinberg equilibrium(HWE)in seven sheep breeds(P>0.05).The g.41871219 T>C locus was under HWE in Small-tail Han sheep,Tan sheep,Sunite sheep,Cele Black sheep and Tibetan sheep.The association analysis showed that there was a significant correlation between the polymorphism of g.41871219 T>C locus and the first parity litter size of Small-tail Han sheep,the litter size of TT was significantly higher than CC(P<0.05),while the g.36651870 T>C locus had no significant correlation with the litter size of the first three parities in Small-Tail Han sheep(P>0.05).In conclusion,the g.41871219 T>C locus of PLCB3 gene may provide a valuable reference to litter size trait selection in Small-tail Han sheep and the g.36651870 T>C locus of SPP1 gene may not suitable for breeding of Small-tail Han sheep. |
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| Keywords: | sheep SPP1 gene PLCB3 gene litter size SNP genotyping |
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