| L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究 |
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| 引用本文： | 陈美艳,刘芬,林勇,左高隆,左颖鹏,刘仲华.L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究[J].茶叶科学,2021,41(4):511-524. |
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| 作者姓名： | 陈美艳 刘芬 林勇 左高隆 左颖鹏 刘仲华 |
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| 作者单位： | 1.湖南农业大学茶学教育部重点实验室,湖南 长沙 410128; 2.国家植物功能成分利用工程技术研究中心,湖南 长沙 410128; 3.湖南省植物功能成分利用协同创新中心,湖南 长沙 410128 |
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| 基金项目： | 湖南省自然科学基金项目（2020JJ4351、2017JJ3106）、湖南省教育厅科学研究重点项目（19A223）、湖南省科技特派员创新创业项目（2020NK4260） |
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| 摘 要： | 以SPF级SD雄性大鼠为实验动物,建立慢性不可预见轻度应激（Chronic unpredictable mild stress,CUMS）大鼠模型,给予不同剂量L-茶氨酸灌胃干预,观察L-茶氨酸对CUMS模型大鼠的抑郁样行为、海马和肠道组织病理变化,以及白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）、5-羟色胺（5-HT）和胰高血糖素样肽1（GLP-1）含量的影响,探讨L-茶氨酸干预对CUMS抑郁大鼠海马和肠道损伤及功能异常的作用。结果表明,相比模型组,L-茶氨酸各剂量组大鼠体质量、糖水偏好、横穿格数和站立次数、水中的活跃度、进入明箱次数及停留时间均有不同程度的改善,中剂量组（100 mg·kg-1）达到显著水平;L-茶氨酸有效修复了CUMS造成的海马神经元损伤,降低了CUMS大鼠血清、结肠和盲肠中的IL-6和TNF-α含量,并减轻结肠、盲肠组织的损伤和炎症程度,且以中剂量组效果较好;L-茶氨酸能增加CUMS大鼠海马、大脑皮层以及结肠中5-HT、GLP-1的含量,部分条件下达到显著水平。由此可见,L-茶氨酸具有改善CUMS模型大鼠抑郁样行为、修复海马和肠道结构损伤以及功能异常等作用,其对“肠-脑轴”的调节可能是抗抑郁的作用机制。
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| 关 键 词： | L-茶氨酸 抑郁样行为 海马 肠道损伤 肠-脑轴 |
| 收稿时间： | 2020-11-02 |
Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats |
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| CHEN Meiyan,LIU Fen,LIN Yong,ZUO Gaolong,ZUO Yingpeng,LIU Zhonghua.Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats[J].Journal of Tea Science,2021,41(4):511-524. |
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| Authors: | CHEN Meiyan LIU Fen LIN Yong ZUO Gaolong ZUO Yingpeng LIU Zhonghua |
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| Institution: | 1. Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China; 2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China; 3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China |
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| Abstract: | Using SPF-grade SD male rats as experimental animals, a rat model of chronic unpredictable mild stress (CUMS) was established and different doses of L-theanine were given intragastrically for intervention. Then, the effects of L-theanine on the depression-like behaviors, histopathological changes and the levels of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), 5-hydroxytryptamine (5-HT) and glucagon-like peptide 1 (GLP-1) contents of hippocampus and gut were observed and evaluated, which aimed to explore the intervention effects of L-theanine on the structural damage and dysfunction of hippocampus and gut in CUMS depressed rats. The results show that, compared with the model group, pretreatment with different doses of L-theanine improved the body weight,sugar-water preference, number of crossing and rearing, activity in water, number of entering the light box and the duration time of ratsat different degrees, especially the middle-dose group (100 mg·kg-1) reached a significant level. L-theanine effectively repaired hippocampal neuron damage caused by CUMS, reduced IL-6 and TNF-α levels in serum, colon and cecum and attenuated the damage and inflammation degree of the colon and cecum tissues, and the middle-dose group also exhibited better effect. L-theanine could increase the contents of 5-HT and GLP-1 in hippocampus, cerebral cortex and colon of CUMS rats, and reached significant levels under some conditions. These results indicate that L-theanine can effectively improve the depression-like behavior, structural damage and dysfunction of hippocampus and gut in CUMS model rats, and its regulation of ‘gut-brain axis’ may be the action mechanism of anti-depression. |
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| Keywords: | L-theanine depression-like behavior hippocampus gut damage gut-brain axis |
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