| 嗜冷乳糖节杆菌B7对遮阴胁迫下多年生黑麦草幼苗生长和抗氧化防御系统的影响 |
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| 引用本文： | 刘鹏,崔彤彤,孙鹏越,周雨,张智伟,尉欣荣,呼天明,付娟娟.嗜冷乳糖节杆菌B7对遮阴胁迫下多年生黑麦草幼苗生长和抗氧化防御系统的影响[J].草业科学,2021,38(1):81-88. |
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| 作者姓名： | 刘鹏 崔彤彤 孙鹏越 周雨 张智伟 尉欣荣 呼天明 付娟娟 |
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| 作者单位： | 西北农林科技大学草业与草原学院,陕西,杨凌,712100 |
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| 基金项目： | 国家自然科学基金项目(31901382)%中国博士后科学基金项目(2018M631208) |
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| 摘 要： | 为探究嗜冷乳糖节杆菌B7 (Arthrobacter psychrolactophilus)对遮阴胁迫下多年生黑麦草(Lolium perenne)的缓解效应，在盆栽试验条件下，研究了不同遮阴胁迫(0、20%、40%、60%和80%)下接种嗜冷乳糖节杆菌菌株B7对黑麦草生长和生理特性的影响。遮阴胁迫显著抑制黑麦草的生长(P < 0.05)，而B7能够有效缓解遮阴胁迫对黑麦草生长的抑制。60%和80%遮阴导致多年生黑麦草细胞膜发生严重的损伤，并且积累较高的活性氧，接种B7有效地缓解了遮阴对黑麦草幼苗造成的氧化损伤。与单独遮阴处理相比，20%、40%、60%和80%遮阴胁迫下接种B7导致H2O2和\begin{document}${\rm{O}}_2^{{\rm{\cdot}} - } $\end{document}含量分别下降5.88%、24.41%、33.60%、40.00%和30.91%、22.94%、25.67%、49.37%。遮阴胁迫在一定程度上提高了超氧化物歧化酶(superoxide dismutase, SOD)、过氧化物酶(peroxidase, POD)、过氧化氢酶(catalase, CAT)、抗坏血酸过氧化物酶(ascorbate peroxidase, APX)、谷胱甘肽还原酶(glutathione reductase, GR)活性以及抗坏血酸(ascorbic acid, AsA)和谷胱甘肽(glutathione, GSH)含量，接种B7进一步提高了上述抗氧化防御体系的活性。研究结果表明，遮阴胁迫下接种B7能够显著提高黑麦草的抗氧化酶活性(P < 0.05)，增加活性氧的清除能力，减轻遮阴引起的氧化损伤，从而增强黑麦草的耐阴性。
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| 关 键 词： | 根际促生菌 嗜冷乳糖节杆菌 遮阴胁迫 活性氧 抗氧化系统 多年生黑麦草 生理响应 |
Effects of Arthrobacter psychrolactophilus B7 on the growth and antioxidant defense system in perennial ryegrass under shade stress |
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| LIU Peng,CUI Tongtong,SUN Pengyue,ZHOU Yu,ZHANG Zhiwei,YU Xinrong,HU Tianming,FU Juanjuan.Effects of Arthrobacter psychrolactophilus B7 on the growth and antioxidant defense system in perennial ryegrass under shade stress[J].Pratacultural Science,2021,38(1):81-88. |
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| Authors: | LIU Peng CUI Tongtong SUN Pengyue ZHOU Yu ZHANG Zhiwei YU Xinrong HU Tianming FU Juanjuan |
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| Abstract: | This study aims to investigate the effects of Arthrobacter psychrolactophilus strain B7 on plant growth and the physiological characteristics of the perennial ryegrass under different shade stress treatments (0, 20%, 40%, 60%, and 80%). The combined effects of B7 inoculation and various levels of shade stress on plant growth and physiological characteristics were investigated via pot experiments. The results showed that shade stress significantly inhibited the perennial ryegrass seedling growth (P < 0.05), whereas B7 inoculation alleviated the growth inhibition caused by shade stress. The relative electrolyte leakage and malondialdehyde accumulations increased with increasing shade stress. B7 inoculation significantly reduced the cellular membrane damage and reactive oxygen species (ROS) levels under shade stress (P < 0.05), compared with shade treatment alone. Inoculation of B7 decreased the H2O2 and \begin{document}${\rm{O}}_2^{{\rm{\cdot}} - } $\end{document} levels by 5.88%, 24.41%, 33.60%, and 40.00%, and by 30.91%, 22.94%, 25.67%, and 49.37% when exposed to 20%, 40%, 60%, and 80% shade stresses, respectively. Additionally, shade stress resulted in an increase in the antioxidant enzyme activities and non-enzyme antioxidant contents, whereas B7 inoculation further improved the activities of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, glutathione reductase, and the contents of ascorbic acid (AsA) and glutathione (GSH). In conclusion, B7 inoculation could improve the antioxidant defense activities, which led to a decline in ROS, thus enhancing shade tolerance in perennial ryegrass. The present study indicates that A. psychrolactophilus strain B7 has considerable potential for improving the ability of plants to adapt to stress. |
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| Keywords: | Plant growth-promoting rhizobacteria Arthrobacter psychrolactophilus shading stress reactive oxygen species antioxidant system perennial ryegrass physiological response |
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