| 多胺氧化降解在莴苣种子萌发过程中的作用(摘要)(英文) |
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| 引用本文： | 王立伟,孙娜,张凤芝,苏国兴.多胺氧化降解在莴苣种子萌发过程中的作用(摘要)(英文)[J].农业科学与技术,2010,11(1):86-89. |
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| 作者姓名： | 王立伟 孙娜 张凤芝 苏国兴 |
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| 作者单位： | 苏州大学基础医学与生物科学学院植物科学系,江苏苏州,215123 |
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| 摘 要： | 目的]了解多胺氧化降解在莴苣种子萌发过程中的作用。方法]用1.0mmol/L多胺氧化酶专性抑制剂氨基胍(AG)在24℃黑暗环境下对莴苣浸种4h,播种于含0.5%琼脂的固体培养基上用于种子萌发率的测定,另播种于用处理试剂湿润的纱布上用于内源多胺、多胺氧化酶(PAO)和二胺氧化酶(DAO)活性以及H2O2含量测定。种子萌发率采用目测法,以胚根突破种皮大于1mm为准。以测定平均值±SE(n≥200)表示种子萌发率的大小;DAO、PAO活性测定参照Su等的方法;多胺高效液相色谱测定参照Flores和Galstonl的方法;H2O2含量测定参照Brennan和Frenkel的方法。结果]AG可强烈抑制种子的萌发,12h后种子萌发率与对照相比差异就达极显著水平。随着莴苣种子的萌发,PAO和DAO活性发生显著的变化,2种酶活性都是先上升后下降,在24和48h分别测得DAO和PAO活性高峰。AG对DAO和PAO活性有强烈的抑制作用,培养24和36h后就检测不到DAO和PAO活性。在种子萌发过程中,内源Put在前24h快速降低,24～60h降低趋缓;Spd在萌发前期降低缓慢,48h后急剧下降;Spm含量低但略呈增加趋势;多胺总量逐渐降低。AG处理可显著增加内源多胺尤其是Put和Spd含量。在种子萌发过程中,种子H2O2含量逐渐增高,36～48h时出现高峰后一直维持较高水平。AG处理可明显降低种子H2O2含量。结论]在种子萌发期间,内源多胺和H2O2含量的变化与PAO和DAO活性变化相对应,暗示在莴苣种子萌发期间进行着活跃的多胺氧化降解代谢。
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| 关 键 词： | 莴苣 种子萌发 多胺降解 多胺氧化酶 |
The Role of Polyamine Oxidative Degradation in the Process of Lettuce Seed Germination |
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| WANG Li-wei,SUN Na,ZHANG Feng-zhi,SU Guo-xing.The Role of Polyamine Oxidative Degradation in the Process of Lettuce Seed Germination[J].Agricultural Science ＆ Technology,2010,11(1):86-89. |
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| Authors: | WANG Li-wei SUN Na ZHANG Feng-zhi SU Guo-xing |
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| Institution: | Department of Plant Sciences,School of Basic Medical and Biological Sciences,Suzhou University,Suzhou 215123 |
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| Abstract: | Objective]The aim of this study was to understand the role of polyamine oxidative degradation in the process of lettuce seed germination. Method]After lettuce seed soaking treatments with aminoguanidine （AG,a specific inhibitor of polyamine oxidases）,seed germination rate,activities of polyamine oxidase （PAO） and diamine oxidase （DAO）,change of endogenous polyamine and H2O2 content were determined. Result]Compared with the control,AG treatment strongly inhibited the seed germination,which also had an extremely significant difference in seed germination rate after incubation for 12 h. During the seed germination,activities of PAO and DAO significantly changed,while their activities firstly increased and then decreased,then DAO and PAO reached peaks at 24 h and 48 h respectively. AG treatment was strongly inhibitory for activities of DAO and PAO,whose activities even disappeared after incubation with AG for 24 h and 36 h. During the seed germination,endogenous Put reduced sharply in the first 24 h,then reduced slowly in 24-60 h,while Spd decreased slowly in the early stage of germination,and then sharply declined after 48 h. However,Spm content was low but slightly increased,and the total polyamine gradually decreased. AG treatment could significantly increase endogenous polyamines,especially Put and Spd contents. During the seed germination,H2O2 content gradually increased,and had a peak from 36 to 48 h,then kept a high level at last. AG treatment could significantly reduce H2O2 content. Conclusion]During the seed germination,the changes of endogenous polyamine and H2O2 content correspond with the changes of PAO and DAO activities,which indicate that there is an active polyamine metabolism of oxidative degradation during the lettuce seed germination. |
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| Keywords: | Lettuce Seed germination Polyamine degradation Polyamine oxidases |
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