| 用B型超声波导引活体采集奶水牛的卵母细胞 |
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| 引用本文： | 覃广胜,陈明堂,李忠权,梁贤威.用B型超声波导引活体采集奶水牛的卵母细胞[J].中国牛业科学,2005,31(2):28-29,37. |
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| 作者姓名： | 覃广胜 陈明堂 李忠权 梁贤威 |
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| 作者单位： | 中国农科院广西水牛研究所,广西,南宁,530001 |
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| 基金项目： | 中国博士后科技基金会资助项目 |
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| 摘 要： | 本试验利用B型超声波导引活体采集42头奶水牛的卵母细胞,每头牛每周采卵2次,共采卵96次,卵母细胞回收率67%,可用卵母细胞数平均为3.1,成熟培养的囊胚率平均为24.7%.对水牛的卵巢观察结果表明活采对水牛的健康及生殖机能不产生影响.
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| 关 键 词： | 活体采集 卵母细胞 采卵 奶水牛 B型超声波 囊胚率 成熟培养 导引 观察 健康 |
| 文章编号： | 1001-9111(2005)02-0028-02 |
Transvaginal Ultrasound-guided Follicular Aspiration of the Diary Buffalo Oocyte Recovery |
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| QIN Guang-sheng,CHEN Ming-tang,LI Zhong-quan,LIANG Xian-wei.Transvaginal Ultrasound-guided Follicular Aspiration of the Diary Buffalo Oocyte Recovery[J].China Cattle Science,2005,31(2):28-29,37. |
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| Authors: | QIN Guang-sheng CHEN Ming-tang LI Zhong-quan LIANG Xian-wei |
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| Abstract: | Forty-two dairy buffaloes were used to collect oocytes from ovaries by Bultrasound-guided follicular aspiration(ovum pick up, OPU). Ovum Collection can be done twice a week without side effects on the reproductive performance. Total times were over 100 times. The results showed that the rate of oocyte recovery, an average of reliable oocytes and blastocyst rate were 67%, 3.1 and 24.7% respectively. |
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| Keywords: | buffalo ovum pick up ultrasound-guide |
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