A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts |
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Authors: | Rebecca Bart Mawsheng Chern Chang-Jin Park Laura Bartley Pamela C Ronald |
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Institution: | (1) Department of Plant Pathology, University of California at Davis, Davis, California, USA |
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Abstract: | Background Transient assays using protoplasts are ideal for processing large quantities of genetic data coming out of hi-throughput assays.
Previously, protoplasts have routinely been prepared from dicot tissue or cell suspension cultures and yet a good system for
rice protoplast isolation and manipulation is lacking. |
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Keywords: | |
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