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A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts
Authors:Rebecca Bart  Mawsheng Chern  Chang-Jin Park  Laura Bartley  Pamela C Ronald
Institution:(1) Department of Plant Pathology, University of California at Davis, Davis, California, USA
Abstract:

Background  

Transient assays using protoplasts are ideal for processing large quantities of genetic data coming out of hi-throughput assays. Previously, protoplasts have routinely been prepared from dicot tissue or cell suspension cultures and yet a good system for rice protoplast isolation and manipulation is lacking.
Keywords:
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