| 水稻miRNA应答低能N+束辐照的基因芯片分析 |
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| 引用本文： | 周晓君,刘旭昊,郭向萌.水稻miRNA应答低能N+束辐照的基因芯片分析[J].安徽农业科学,2011,39(5):2567-2569. |
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| 作者姓名： | 周晓君 刘旭昊 郭向萌 |
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| 作者单位： | 洛阳师范学院生命科学系; |
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| 基金项目： | 国家自然科学基金项目(308200204); 河南省教育厅自然科学研究计划项目(2009B180019) |
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| 摘 要： | 目的]探讨水稻应答N＋束辐照机制中相关miRNA的表达差异。方法]各选取3组N＋束辐照处理水稻种子和未处理的水稻种子,提取其在30℃发芽96 h幼苗总RNA,采用Agilent单通道水稻基因表达谱芯片筛选2组水稻幼苗差异表达基因。芯片中含有miRNA探针510个,分析其在样品间表达差异2倍左右作为域值范围。结果]辐照组与对照组相比表达差异显著的miRNA分子有14个,且均为下调。结论]水稻N＋束辐照组和对照组中存在差异表达的miRNA分子,可能调控某些基因的表达来应答离子辐照,从而表现出与对照不一样的生理生化和表型差异。
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| 关 键 词： | N＋束 辐照水稻 miRNA 差异表达 基因芯片 |
Gene Chip Analysis for Rice MicroRNA Response to Low Energy N~+ Beam Radiation |
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| ZHOU Xiao-jun et al.Gene Chip Analysis for Rice MicroRNA Response to Low Energy N~+ Beam Radiation[J].Journal of Anhui Agricultural Sciences,2011,39(5):2567-2569. |
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| Authors: | ZHOU Xiao-jun |
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| Institution: | ZHOU Xiao-jun et al (Department of Life Sciences,Luoyang Normal University Luoyang,Henan 471022) |
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| Abstract: | Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N+ beam radiation in rice.Method] Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.Result] 14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.Conclusion] The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences. |
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| Keywords: | Nitrogen ion beam Irradiated rice MiRNA Differential expression Gene chip |
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