| 转基因牛与非转基因牛性控精液分离及配种效果初探 |
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| 引用本文： | 吕小青,朱玉林,李艳华,薛建华,杜欣悦,王彦平,戴蕴平.转基因牛与非转基因牛性控精液分离及配种效果初探[J].中国奶牛,2012(13):37-39. |
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| 作者姓名： | 吕小青 朱玉林 李艳华 薛建华 杜欣悦 王彦平 戴蕴平 |
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| 作者单位： | [1]北京奶牛中心,北京100192 [2]中国农业大学生物学院,北京100193 |
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| 基金项目： | “高品质转基因奶牛新品种培育（2011ZX08007-001）”资助. |
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| 摘 要： | 本研究对转基因牛以及非转基因牛精液经流式细胞仪分离冷冻后精子活力、分离准确率进行了比较,同时对分离的性控冷冻精液进行了人工输精,对受体牛的情期受胎率进行了统计分析。结果表明,转基因牛与非转基因牛精液在冷冻解冻后活力以及分离准确率方面差异不显著(P>0.05);转基因牛与非转基因牛的性控冷冻精液的情期受胎率分别为57.4%、59.3%,二者之间差异不显著(P>0.05)。
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| 关 键 词： | 转基因牛 性控精液 配种效果 |
Preliminary Analysis of Sexed Semen Separation and Breeding Results of Transgenic and Non-transgenic Cattle |
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| LU Xiao-qing,ZHU Yu-lin,LI Yan-hua,XUE Jian-hua,DU Xin-yue,WANG Yan-ping,DAI Yun-ping.Preliminary Analysis of Sexed Semen Separation and Breeding Results of Transgenic and Non-transgenic Cattle[J].China Dairy Cattle,2012(13):37-39. |
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| Authors: | LU Xiao-qing ZHU Yu-lin LI Yan-hua XUE Jian-hua DU Xin-yue WANG Yan-ping DAI Yun-ping |
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| Institution: | 1. Beijing Dairy Cattle Center, Beijing 100192; 2. College of Biological Sciences, China Agricultural University, Beijing 100193) |
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| Abstract: | In this study, we analysed the difference of sperm motility and X/Y ratio of sorted sperm between transgenic and non-transgenic dairy cow sperm separation after freezing and thawing, and also analysed the recipient cow pregnancy rate after sorted semen artificial insemination. The results showed that the sperm motility and X/Y ratio of sorted sperm did not differ significantly between transgenic cattle and non-transgenic cattle; the pregnancy rate of non-transgenic cattle and transgenic cattle sexed semen were 59.3% （non-transgenic cattle） and 57.4% （transgenic cattle） respectively, there were no significant differences（P 〈0.05）. |
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| Keywords: | Transgenic cattle Sexed semen Breeding results |
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