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液相阻断ELISA检测方法在猪牛A型口蹄疫免疫抗体检测中的应用
引用本文:熊东艳,葛忠源.液相阻断ELISA检测方法在猪牛A型口蹄疫免疫抗体检测中的应用[J].北京农业,2011(Z1):19-22.
作者姓名:熊东艳  葛忠源
作者单位:北京市平谷区动物疫病预防控制中心;北京市平谷区动物卫生监督所
摘    要:用液相阻断ELISA试验方法监测辖区内经口蹄疫A型灭活疫苗免疫牛血清样品593份,其中:规模场牛群免疫抗体滴度<1∶64的有24份,抗体滴度≥1∶64的有82份,抗体保护率为77.79%;散养户牛群免疫抗体滴度<1∶64的有54份,抗体滴度≥1∶64的有433份,抗体保护率为88.91%。在猪群推广免疫口蹄疫A型灭活疫苗并跟踪监测血清样品20份,检测结果与北京市兽医兽医诊断所检测结果相同,抗体滴度≥1∶64的有20份,抗体保护率为100.00%,为推广口蹄疫A型灭活疫苗在猪群免疫提供了试验数据。

关 键 词:    液相阻断ELISA  A型口蹄疫  抗体

Liquid Block ELISA Test Methods in the Gig, Cattle Type A Foot-and-mouth Disease Immune Antibody Test Applications
Xiong Dongyan Ge Zhongyuan.Liquid Block ELISA Test Methods in the Gig, Cattle Type A Foot-and-mouth Disease Immune Antibody Test Applications[J].Beijing Agriculture,2011(Z1):19-22.
Authors:Xiong Dongyan Ge Zhongyuan
Institution:Xiong Dongyan Ge Zhongyuan
Abstract:With liquid block ELISA test method monitoring jurisdiction type A classic foot-and-mouth disease inactivated vaccine immune bovine serum sample of 593, the number of scale cattle immune antibody drops A degree for 4 with 24 "of antibody drops, for the 4: degree of 82, the BaoHuLv antibodies have to 77.79%; Cattle keeping free-roaming households immune antibody drops for the 4 "degree of 54, antibody drops for the 4 a: degree of 433, the BaoHuLv antibody is 88.91%. In pig group of promotion immune foot- and-mouth disease type A inactivated vaccine and track monitoring serum sample of 20, the testing results and the Beijing veterinary veterinary diagnosis are same, antibody test results for the 4: drop degrees 20, 100.0% of BaoHuLv antibody, to promote the foot-and- mouth disease type A inactivated vaccine in pig group of immune provides the test data.
Keywords:cattle  pigs  liquid block ELISA  type A foot-and-mouth disease  antibody
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