| 忍冬和华南忍冬叶表皮扫描电镜观察及其生态适应性研究 |
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| 作者姓名： | LI Qiang ;YU Long-jiang ;DENG Yan ;LI Wei ;LI Mao-teng ;CAO Jian-hua |
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| 作者单位： | [1]School of Life Science and Technology, Huazhong University of Science and Technology Wuhan 430074, P. R. China; [2]Karst Dynamics Laboratory. Institute of Karst Geology Chinese Academy of Geological Sciences Guilin 541004, P. R. China |
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| 摘 要： | 利用扫描电镜观察了忍冬（Lonicera jàponica Thunb）和华南忍冬（Lonicera confus）叶表皮形态特征,观察指标包括：气孔器、表皮毛、表皮细胞等.结果表明：气孔仅分布在下表皮,气孔器呈无规则型散生;上表皮细胞的垂周壁呈沟槽状下陷;下表皮密被表皮毛（包括腺状毛和非腺毛）,同时还分布有瘤状的草酸钙簇晶.为进一步证明这种瘤状结构是否为草酸钙簇晶,作者发现利用Fluo-3/AM（钙离子荧光指示剂）处理的活体叶片在激光共聚焦显微镜下能够发出强烈的荧光.此外,通过电子探针对瘤状物进行分析,发现其元素组成为C、O、Ca.上述叶表皮构造特征,有利于减少植物体水分散失,还有助于植物适应富钙的石灰土,从而适应我国西南岩溶区特殊的干旱、富钙的环境.电镜观察结果表明,在忍冬叶上表皮中脉处有呈线状分布的腺状表皮毛,而华南忍冬叶上表皮则无表皮毛分布,这一微形态特征可以作为二者种间特异性差别,从而为二者的合理区分提供一定的依据.
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| 关 键 词： | 叶表皮细胞 气孔器 表皮毛 生态适应 忍冬 华南忍冬 忍冬属 |
| 收稿时间： | 17 March 2010 |
Leaf epidermal characters of <Emphasis Type="Italic">Lonicera japonica</Emphasis> and <Emphasis Type="Italic">Lonicera confuse</Emphasis> and their ecology adaptation |
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| LI Qiang,;YU Long-jiang,;DENG Yan,;LI Wei,;LI Mao-teng,;CAO Jian-hua.Leaf epidermal characters of Lonicera japonica and Lonicera confuse and their ecology adaptation[J].Journal of Forestry Research,2007,18(2):103-108. |
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| Authors: | Li Qiang Yu Long-jiang Deng Yan Li Wei Li Mao-teng Cao Jian-hua |
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| Institution: | (1) School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China;(2) Karst Dynamics Laboratory, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin, 541004, P. R. China |
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| Abstract: | The leaf epidermis of Japanese honeysuckle (Lonicera japonica Thunb.) and Wild Honeysuckle (Lonicera confusa) in the genus of Flos Lonicerae were mainly observed by scanning electron microscopes (SEM) to study the characteristics of stomata, trichomes and dermal
cell, etc.. The results showed that stoma exists only on the lower epidermis and its distribution is irregular, and leaf epidermis consist
of epidermis cells, stoma complexes and bushy trichomes including glandular hair and non-glandular hair. On the upper epidermis,
anticlinal wall caves in sinuous groove to countercheck the transpiration. Evidences from leaf morphological structures serve
as another proof on drought-resistant mechanisms. Some strumaes distributing regularly are hypothesized as oxalic calcium
on the lower epidermis under laser scanning confocal microscopy (LSCM) with Fluo-3/AM, which can increase their endurance
to drought stress. Therefore, the above characteristics of Flos Lonicerae can reduce the loss of water and make Japanese honeysuckle and Wild Honeysuckle adapt to the droughty environment at Karst
area in southwest China. However, there is some difference of the two species. From the SEM (Scanning Electron Microscopy)
result, it is shown that on the upper epidermis, some glandular hair regularly present along the midrib of Japanese honeysuckle,
but Wild Honeysuckle has no glandular hair on the upper epidermis, which can verify the relationships of Flos Lonicerae species and provide the significance for classification of Flos Lonicerae.
Foudation project: This study was supported by the Ministry of Sciences and Technology of China (No.2005DIB3J067), the National
Science Foundation of China (No.40572107, No.40231008, No.40672165 and No.30600074), the Chongqing Science & Technology Commission
(No.2005AB7006), the Open Fund and Key Subject of Physical Geography, Southwest Normal University of China (No.250-411110)
and the Open Fund of Key Laboratory of Chinese Academy of Geological Sciences (No.KL05-20).
Electronic supplementary material is available in the online version of this article at http://dxdoi.org/10.1007/s11676-007-0020-1
Biography: LI Qiang (1978–), * Corresponding author, male, Ph.D. in School of Life Science and Technology, Huazhong University
of Science and Technology, Wuhan 430074, P. R. China. |
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| Keywords: | Lonicera japonica Lonicera confuse Flos Lonicerae Dermal cell Stomata Trichomes Ecology adaptation |
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