| 荧光假单胞菌GcM5-1A胞外木质素过氧化物酶的初步纯化及性质研究 |
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| 引用本文： | 孔令营,郭道森,赵博光,李荣贵.荧光假单胞菌GcM5-1A胞外木质素过氧化物酶的初步纯化及性质研究[J].北京林业大学学报,2010,32(3):112-116. |
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| 作者姓名： | 孔令营 郭道森 赵博光 李荣贵 |
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| 作者单位： | 1 青岛大学生物系 2 南京林业大学森林资源与环境学院 |
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| 基金项目： | 国家自然科学基金重点项目 |
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| 摘 要： | 本文研究了松材线虫携带的荧光假单胞菌GcM5-1A的培养液经硫酸铵分级沉淀后,各组分中木质素过氧化物酶(LiP)的活性及各组分对黑松悬浮细胞的毒性;还对GcM5-1A菌株胞外LiP进行了初步纯化,并对纯化后的酶学性质进行了研究。结果表明:LiP活性主要出现在50%～70%沉淀组分中;0～20%沉淀组分和50%～70%沉淀组分对黑松悬浮细胞的毒性很强;GcM5-1A菌株培养液经硫酸铵分级沉淀以及DEAE-SepharoseFF两步纯化后,其LiP的比活力从0.81U/mg提高到21.30U/mg;其最适温度为35℃,最适pH为4.0;在15～35℃、pH6.0～9.0范围内,酶活力保持相对稳定;NH2OH·HCl和KCN对酶活力的抑制作用分别达到88%和57%,而NaN3对酶活力的抑制作用只有15%左右;吸收光谱显示该酶在406nm处有一个Soret峰,加入1mmol/LNa2S2O4后,吸光度出现了明显的下降,但Soret峰没有显著移动。
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| 关 键 词： | 松材线虫 荧光假单胞菌 木质素过氧化物酶 毒素 |
| 收稿时间： | 1900-01-01 |
Preliminary purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM5-1A |
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| KONG Ling-ying,GUO Dao-sen,ZHAO Bo-guang,LI Rong-gui.Preliminary purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM5-1A[J].Journal of Beijing Forestry University,2010,32(3):112-116. |
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| Authors: | KONG Ling-ying GUO Dao-sen ZHAO Bo-guang LI Rong-gui |
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| Institution: | 1 Department of Biology, Qingdao University, Shandong Province, 266071, P.R. China; 2 College of Forest Resources and Environment, Nanjing Forestry University, 210037, P.R. China. |
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| Abstract: | In this study,a fermented culture of Pseudomonas fluorescens GcM5-1A carried by the pinewood nematode,Bursaphelenchus xylophilus,was precipitated with ammonium sulfate solutions with different saturation rates and the resulting solution was used to test lignin peroxidase(LiP) activity and its toxicity to a suspension of Pinus thunbergii cells.We also studied the preliminary purification and characterization of extracellular LiP from P.fluorescens GcM5-1A.The results show that LiP activity mainly existed in ... |
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| Keywords: | pine wood nematode disease Pseudomonas fluorescens lignin peroxidase toxin |
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