| 北疆地区猪繁殖与呼吸综合征病毒ORF7基因克隆与遗传演化分析 |
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| 引用本文： | 吴鹏,张勋,梁田,陈新凯,肖媛媛,盛金良.北疆地区猪繁殖与呼吸综合征病毒ORF7基因克隆与遗传演化分析[J].新疆农业科学,2016,53(11):2149. |
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| 作者姓名： | 吴鹏 张勋 梁田 陈新凯 肖媛媛 盛金良 |
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| 作者单位： | 石河子大学动物科技学院，新疆地方与民族高发病教育部重点实验室，新疆石河子 832003 |
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| 摘 要： | 【目的】研究2014年新疆地区猪繁殖与呼吸综合征病毒（PRRSV）遗传变异情况，监测PRRSV在新疆流行情况，为新疆的PRRSV血清学检测提供帮助。【方法】采用RT－PCR方法对分离自新疆地区的PRRS疑似病料进行ORF7基因扩增和测序，分析PRRSV ORF7基因变异情况。【结果】检测到18株PRRSV毒株具有ORF7碱基突变，均属北美洲型，与国外的参考毒株和国内的参考毒株有着不同的亲缘关系。其中，XJzx4、XJzx12与国外的参考毒株01NP1.2、PA8、VR－2332、LMY亲缘关系较近。除XJzx1、XJzx14外，其余毒株与内地毒株BJsy06、GD、HB－1 sh2002、HuN、JXA1、LN、NX06、SX2009亲缘关系较近，推断由内地毒株演化而来。【结论】新疆本地流行猪繁殖与呼吸综合征毒株是来自国内外不同地区毒株，ORF7基因在新疆具有不同的遗传变异类型。没有检测到新疆猪繁殖与呼吸综合征病毒ORF7基因有缺失型突变。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 RT-PCR ORF7 序列分析 |
| 收稿时间： | 2016-11-25 |
PRRSV ORF7 Cloning and Genetic Evolution Analysis of Pigs in Northern Xinjiang |
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| WU Peng,ZHANG Xun,LIANG Tian,CHEN Xin-kai,XIAO Yuan-yuan,SHENG Jin-liang.PRRSV ORF7 Cloning and Genetic Evolution Analysis of Pigs in Northern Xinjiang[J].Xinjiang Agricultural Sciences,2016,53(11):2149. |
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| Authors: | WU Peng ZHANG Xun LIANG Tian CHEN Xin-kai XIAO Yuan-yuan SHENG Jin-liang |
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| Abstract: | Objective]To study porcine reproductive and respiratory syndrome virus (PRRSV)genetic variation to monitor the prevalence of PRRSV in Xinjiang and provide assistance to PRRSV serology.Method]We used RT-PCR method for the separation of 18 PRRSV ORF7 gene which was amplified,cloned and se-quenced to analyze the ORF7 gene variationResults]The 18 PRRSV ORF7 sequence mutation type belonged to North America type.They had different genetic strain relations with the international standards and national standards strains.XJzx 4 and XJzx 1 had a closer genetic relationship with the international standard strains 01NP1.2,PA8,VR-2332 and LMY.Except XJzx1,XJzx14,the rest strains had close relationships with the mainland strains BJsy06,GD,HB-1sh 2002,HuN,JXA1,LN,NX06 and SX2009,so it could be inferred that they evolved from the strains in mainland China.Conclusion]It is hypothesized that local pop PRRSV strains are derived from different regions.We have not detected PRRSV ORF7 gene deletion mutation in Xin-jiang. |
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