| 菜籽蛋白质组双向电泳技术体系的优化研究(英文) |
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| 引用本文： | 王丽娟,任学敏,杜艳慧,张其彬,罗水忠,姜绍通,郑志.菜籽蛋白质组双向电泳技术体系的优化研究(英文)[J].Agricultural Science&Technology,2011(5). |
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| 作者姓名： | 王丽娟 任学敏 杜艳慧 张其彬 罗水忠 姜绍通 郑志 |
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| 作者单位： | 合肥工业大学生物与食品工程学院安徽省农产品精深加工重点实验室; |
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| 基金项目： | Supported by National 863 Project(2010AA101503); National Science and Technology Support Planning Item(2006BAD05A12); Student Innovation Fund Item of Hefei University of Technology(XS2010100)~~ |
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| 摘 要： | 目的]建立适用于菜籽蛋白质组研究的双向电泳技术。方法]以湘油17号为材料,对双向电泳技术中的样品制备方法、凝胶浓度、上样量等条件进行了优化。结果]使用TCA-丙酮法提取菜籽总蛋白最佳,所获蛋白在2-DE图谱上获得的点数最多。当采用pH3～10IPG胶条和12%的凝胶、上样量为250μg时,可得到背景清晰、重复性好、蛋白点分辨率较高的双向电泳图谱。结论]为开展油菜种子蛋白质组学研究奠定了基础。
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| 关 键 词： | 菜籽 蛋白质组 双向电泳 体系优化 |
Study on the Optimization of Two-dimensional Electrophoresis Technology System for Rapeseed Proteome |
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| WANG Li-juan,REN Xue-min,DU Yan-hui,ZHANG Qi-bin,LUO Shui-zhong,JIANG Shao-tong,ZHENG Zhi Key Laboratory for the Intensive Processing of Agricultural Products in Anhui Province,College of Biotechnology , Food Engineering,Hefei University of Technology,Hefei.Study on the Optimization of Two-dimensional Electrophoresis Technology System for Rapeseed Proteome[J].农业科学与技术(英文版),2011(5). |
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| Authors: | WANG Li-juan REN Xue-min DU Yan-hui ZHANG Qi-bin LUO Shui-zhong JIANG Shao-tong ZHENG Zhi Key Laboratory for the Intensive Processing of Agricultural Products in Anhui Province College of Biotechnology Food Engineering Hefei University of Technology Hefei |
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| Institution: | WANG Li-juan,REN Xue-min,DU Yan-hui,ZHANG Qi-bin,LUO Shui-zhong,JIANG Shao-tong,ZHENG Zhi Key Laboratory for the Intensive Processing of Agricultural Products in Anhui Province,College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009 |
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| Abstract: | Objective] The research aimed to establish the two-dimensional electrophoresis(2-DE)technology which was suitable for the rapeseed proteome research.Method] Xiangyou 17 was as the material.The sample preparation method,gel concentration and loading amount,etc.in 2-DE technology were optimized.Result] The best extraction method of total protein of rapeseed was TCA-acetone method,and the protein spots on 2-DE map were the most.When IPG strip(pH 3-10)and 12% gel were used,and the loading amount was 250 μg,the two-dimensional electrophoresis map with the clear background,good repeatability and high protein spot resolution was obtained.Conclusion] The research laid the foundation for carrying out the rapeseed proteomics research. |
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| Keywords: | Rapeseed Proteome Two-dimensional electrophoresis System optimization |
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