| 香蕉中一个新的S-腺苷甲硫氨酸合成酶基因的克隆及采后表达分析 |
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| 引用本文： | 贾彩红,林水玉,张建斌,刘菊华,金志强,徐碧玉.香蕉中一个新的S-腺苷甲硫氨酸合成酶基因的克隆及采后表达分析[J].果树学报,2009,26(3). |
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| 作者姓名： | 贾彩红 林水玉 张建斌 刘菊华 金志强 徐碧玉 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海口,571101
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金 |
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| 摘 要： | 利用抑制消减文库从香蕉中分离到一个cDNA片断,结合RACE技术获得该基因的全长序列。该全长cDNA共含1 285个碱基,通过Blastx同源性分析结果显示它与香蕉的一个S-adenosyl-L-methionine synthase(SAMS)基因(GenBank序列号为AF004317)具有82%的碱基同源性,编码的氨基酸顺序有93%同源性,但在cDNA的5’和3’非编码区同源性较低。设计特异引物对此基因进行RT-PCR分析表明,正常成熟的香蕉果实,采后当天表达量较高,随后略有降低,至采后12 d又达到一个相对较高值后迅速下降;高锰酸钾处理的果实,整个成熟期该基因均表现一个比较高的表达量;乙烯利处理的果实,采后表达量明显下降且处在一个比较稳定的水平。
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| 关 键 词： | 香蕉 S-腺苷甲硫氨酸合成酶基因 克隆 表达分析 |
Cloning and characterization of a Novel S-Adenosyl-L-Methionine Synthase gene in banana |
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| JIA Cai-hong,LIN Shui-yu,ZHANG Jian-bin,LIU Ju-hua,JIN Zhi-qiang,XU Bi-yu.Cloning and characterization of a Novel S-Adenosyl-L-Methionine Synthase gene in banana[J].Journal of Fruit Science,2009,26(3). |
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| Authors: | JIA Cai-hong LIN Shui-yu ZHANG Jian-bin LIU Ju-hua JIN Zhi-qiang XU Bi-yu |
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| Institution: | Institute of Tropical Biotechnology;Chinese Academy of Tropical Agricultural Sciences;Haikou;Hainan 570011 China |
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| Abstract: | SSH was used to identify a novel gene in banana plants.A full length cDNA of S-adenosyl-L-methionine synthase which contained 1 258 bp was isolated by this way combined with RACE technology.Sequence analysis revealed that this gene shared 82% similarity in gene sequence and 93% similarity in protein sequence with a known SAMS(AF004317) gene though they had low homology in the 5’and 3’uncoden regions.RT-PCR analysis indicated that in naturally ripened fruit,the expression was in a relative high level at the day of harvest(0 day after harvest),and then decreased slightly.It reaches a highest level on 12 d followed by a sharply decline.When treated with potassium permanganate,the SAMS gene expression remained high throughout the ripening stage,but in fruit treated by ethylene,the expression decreased significantly and maintained a low level. |
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| Keywords: | Banana S-Adenosyl-L-Methionine synthase Cloning Characterization |
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