Resistance phenotypes and genotypes among multiple-antimicrobial-resistant
Salmonella enterica subspecies enterica serovar
Choleraesuis strains isolated between 2008 and 2012 from slaughter pigs in Okinawa
Prefecture,Japan |
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| Authors: | Masanao MATAYOSHI Takashi KITANO Tetsu SASAKI Masaji NAKAMURA |
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| Institution: | 1)Okinawa Prefecture Central Livestock Hygiene Service Center, 2505 Ozato, Ozato, Nanjyo, Okinawa 901–1202, Japan;2)Okinawa Prefecture Central Meat Inspection Center, 2015 Ozato, Ozato, Nanjyo, Okinawa 901–1202, Japan |
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| Abstract: | A total of 349 Salmonella enterica subspecies enterica
serovar Choleraesuis (S. Choleraesuis) strains, which were isolated
between 2008 and 2012 from 349 pigs at two slaughterhouses in Okinawa Prefecture, Japan,
were investigated for antimicrobial susceptibility and the presence of antimicrobial
resistance genes. All isolates were resistant to at least four antimicrobial agents. The
antimicrobial agents for which isolates showed a high incidence of resistance were as
follows: ampicillin (100%) and streptomycin (100%), followed by gentamicin (99.7%),
oxytetracycline (99.7%), sulfamethoxazole/trimethoprim (99.4%), nalidixic acid (40.1%) and
oxolinic acid (40.1%). All isolates were sensitive to cefuroxime, ceftiofur, colistin,
fosfomycin, enrofloxacin, orbifloxacin and danofloxacin. The predominant resistance
phenotypes and genotypes were: resistance to ampicillin, streptomycin, gentamicin,
oxytetracycline and sulfamethoxazole/trimethoprim (58.5%, 204/349) and
blaTEM-strA-strB-aadA1-aadA2-aacC2-tet
(B)-sul1-sul2-dhfrXII-dhfrXIII (36.1%, 126/349). The quinolone
resistance-determining regions (QRDRs) of gyrA, gyrB, parC and
parE of the quinolone-resistant isolates (n=12) showed amino acid
substitutions of Ser-83→Phe or Asp-87→Tyr in GyrA and Ser-107→Ala in ParC. To our
knowledge, this is the first report on the molecular characterization of antimicrobial
resistance among S. Choleraesuis strains in Japan. |
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| Keywords: | antimicrobial resistance pig resistance genes Salmonella Choleraesuis zoonosis |
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