Genetic and histological characterization of a novel recessive genic male sterile line of <Emphasis Type="Italic">Brassica napus</Emphasis> derived from a cross with <Emphasis Type="Italic">Capsella bursa</Emphasis>-<Emphasis Type="Italic">pastoris</Emphasis> |
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| Authors: | Hai-Feng Chen Xian-Hong Ge Xue-Zhu Du Zhi-Gang Zhao Zai-Yun Li |
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| Institution: | (1) Ministry of Agriculture Key Laboratory for Genetic Improvement of Oil Crops, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 430062 Wuhan, China;(2) National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), Huazhong Agricultural University, 430070 Wuhan, China |
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| Abstract: | In a previously made cross Brassica napus cv. Oro (2n = 38) × Capsella bursa-pastoris (2n = 4x = 32), one F1 hybrid with 2n = 38 was totally male sterile. The hybrid contained no complete chromosomes from C. bursa-pastoris, but some specific AFLP (amplified fragment length polymorphism) bands of C. bursa-pastoris were detected. The hybrid was morphologically quite similar to ‘Oro’ except for smaller flowers with rudimentary stamens
but normal pistils, and showed good seed-set after pollination by ‘Oro’ and other B. napus cultivars. The fertility segregation ratios (3:1, 1:1) in its progenies indicated that the male sterility was controlled
by a single recessive gene. In the pollen mother cells of the male sterile hybrid, chromosome pairing and segregation were
normal. Histological sectioning of its anthers showed that the tapetum was multiple layers and was hypertrophic from the stage
of sporogenic cells, and that the tetrads were compressed by the vacuolated and disaggregated tapetum and no mature pollen
grains were formed in anther sacs, thus resulting in male sterility. The possible mechanisms for the production of the male
sterile hybrid and its potential in breeding are discussed. |
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| Keywords: | Brassica napus Capsella bursa-pastoris Genic male sterility Tapetum AFLP |
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