| 辽宁绒山羊抑制素A基因遗传变异与双羔性状的关联分析 |
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| 引用本文： | 董李学,刘月琴,张英杰,宋杰,程善燕,郭勇庆.辽宁绒山羊抑制素A基因遗传变异与双羔性状的关联分析[J].河北农业大学学报,2010,33(1). |
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| 作者姓名： | 董李学 刘月琴 张英杰 宋杰 程善燕 郭勇庆 |
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| 作者单位： | 河北农业大学,动物科技学院,河北,保定,071000 |
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| 基金项目： | 国家绒毛羊产业技术体系建设(nycytx-40-28) |
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| 摘 要： | 以抑制素A基因(Inhibin alpha subunit gene,INHA)作为辽宁绒山羊双羔性状的候选基因,以便为绒山羊育种工作中分子标记选择提供理论依据。运用测序法寻找辽宁绒山羊INHA基因单核苷酸多态性,筛查到一个新的多态位点-446TC,该位点位于INHA基因的启动子区域。运用PCR-RFLP法验证并分析该位点在辽宁绒山羊双羔群体和单羔群体间的多态性。结果表明,在辽宁绒山羊中双羔和单羔群体中分布A、B两个等位基因,处于中度多态。经χ2检验,样本在该位点未达到Hardy-Weinberg平衡状态(P0.05),不同基因型在辽宁绒山羊单、双胎群体上差异达到显著水平(P0.05)。
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| 关 键 词： | 辽宁绒山羊 INHA基因 双胎性 PCR-RFLP |
Genetic variation of INHA gene and its relationship with twinning trait in Liaoning cashmere goat |
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| DONG Li-xue,LIU Yue-qin,ZHANG Ying-jie,SONG Jie,CHENG Shan-yan,GUO Yong-qing.Genetic variation of INHA gene and its relationship with twinning trait in Liaoning cashmere goat[J].Journal of Agricultural University of Hebei,2010,33(1). |
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| Authors: | DONG Li-xue LIU Yue-qin ZHANG Ying-jie SONG Jie CHENG Shan-yan GUO Yong-qing |
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| Institution: | College of Animal Science and Technology;Agricultural University of Hebei;Baoding 071000;China |
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| Abstract: | Inhibin alpha subunit(INHA) gene was studied as a candidate gene for the twinning trait of Liaoning cashmere goat in order to provide the theoretical basis for breeding work.A new SNP-446T>C was detected by sequencing at promoter.Different genotypes were determined in Liaoning cashmere goat by restriction fragment length polymorphism(RFLP).The value of polymorphism information content indicated that this was a moderate polymorphism in Liaoning cashmere goat.The χ2 test indicated that the polymorphic locus in Liaoning cashmere goat did not fit Hardy-Weinberg equilibrium(P<0.05).The χ2 test of associational analysis between genotypic distribution and twinning or monotocous trait in Liaoning cashmere goat showed that the difference was significant(P<0.05). |
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| Keywords: | Liaoning cashmere goat Inhibin alpha subunit gene twinning trait PCR-RFLP |
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