|
|||||
|
|
| 不同浓度乙二醇和甘油对犬精液冷冻效果的影响 | |
| 引用本文： | 高雅,罗桂河,李卫东,李复煌,孟丹娜,康蕊,秦秀蕊,倪和民.不同浓度乙二醇和甘油对犬精液冷冻效果的影响[J].中国农学通报,2010,26(8):25-28. |
| 作者姓名： | 高雅 罗桂河 李卫东 李复煌 孟丹娜 康蕊 秦秀蕊 倪和民 |
| 作者单位： | 1. 北京农学院动物科技系,北京,102206 2. 北京市昌平区兽医局,北京,102200 3. 北京市昌平区动物卫生防疫站,北京,102200 4. 北京市畜牧兽医总站,北京,100010 |
| 基金项目： | 北京市教育委员会科技发展计划重点项目 |
| 摘 要： | 研究在常规冷冻稀释液中分别添加不同浓度的乙二醇作为保护剂,并与甘油作为冷冻保护剂进行对比,以优化犬精液冷冻方案,提高解冻后精子质量。在犬的常规精液冷冻稀释液中分别添加不同浓度的乙二醇(2%,4%,6%,8%,10%),经细管冷冻、解冻后通过分别评价其精子的活率、顶体完整率、畸形率等来筛选其最佳添加浓度。结果表明:添加浓度为6%的乙二醇时,冷冻后精子活力为0.37,顶体完整率为43%,显著好于其他处理组(P0.05);添加浓度为4%的乙二醇时,冷冻后精子畸形率为15%,略优于其他处理组。6%浓度的乙二醇可代替甘油作为冷冻保护剂,且对犬的冷冻效果有显著的促进作用。 |
| 关 键 词： | 蔬菜 蔬菜 硝酸盐 累积 影响 |
| 收稿时间： | 2009-10-26 |
| 修稿时间： | 2009-11-20 |
Influence of Different Concentrations of Ethylene Glycol and Glycerol on Quality of Canine Frozen Sperm |
|
| Gao Y,Luo Guihe,Li Weidong,Li Fuhuang,Meng Dann,Kang Rui,Qin Xiuru.Influence of Different Concentrations of Ethylene Glycol and Glycerol on Quality of Canine Frozen Sperm[J].Chinese Agricultural Science Bulletin,2010,26(8):25-28. | |
| Authors: | Gao Y Luo Guihe Li Weidong Li Fuhuang Meng Dann Kang Rui Qin Xiuru |
| Institution: | Gao Ya1,Luo Guihe2,Li Weidong3,Li Fuhuang4,Meng Danna2,Kang Rui1,Qin Xiurui1,Ni Hemin1(1Department of Animal Science , Technology,Beijing University of Agriculture,Beijing 102206,2Changping Bureau of Veterinary Medicine of Beijing,Beijing 102200,3Changping Station of Veterinary Medicine , Prevention of Beijing,4General Station of Veterinary Medicine of Beijing Municipal Government,Beijing 100010) |
| Abstract: | To optimize the freezing programs for canine semen,the different concentrations of ethylene glycol as protective agent were added in the conventional frozen diluted solution respectively,and compared with the groups added by glycerol as cryoprotectant.The different concentrations of ethylene glycol(2%,4%,6%,8%,10%) were added in conventional frozen diluted solution of canine semen respectively,the postthaw semen motility,the percentage of sperms with intact acrosome and the percentage of abnormality were re... |
| Keywords: | frozen spermzz |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农学通报》浏览原始摘要信息 | |
| 点击此处可从《中国农学通报》下载免费的PDF全文 | |