| 卷丹的原球茎诱导及植株再生 |
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| 引用本文： | 何微,景丹龙,陈强,王静,张德春.卷丹的原球茎诱导及植株再生[J].湖北农业科学,2012(2):396-399. |
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| 作者姓名： | 何微 景丹龙 陈强 王静 张德春 |
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| 作者单位： | 三峡大学生物技术研究中心;神农架国家级自然保护区管理局生物多样性保护研究实验室 |
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| 基金项目： | 湖北省教育厅科学研究计划项目(2002A53016) |
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| 摘 要： | 以卷丹(Lilium lancifolium)的鳞茎、叶片和珠芽为外植体,建立了卷丹快速繁殖的植株再生体系,探讨了不同外植体、培养基对卷丹原球茎诱导、分化的影响。结果表明,鳞茎是最适合诱导原球茎的外植体,诱导愈伤组织的最佳培养基为MS+1.00 mg/L 6-BA+1.00 mg/L NAA;诱导原球茎的最佳培养基为MS+0.50 mg/L 6-BA+0.10 mg/L NAA+1.00 mg/L 2,4-D;较适合原球茎出芽的培养基为MS+1.00 mg/L 6-BA+0.10 mg/L NAA,较适合不定芽生根的培养基为MS+0.50 mg/L NAA。
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| 关 键 词： | 卷丹 原球茎 诱导 植株再生 |
Research on Inducement of Lilium lancifolium Protocorm and Plant Regeneration |
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| HE Wei,JING Dan-long,CHEN Qiang,WANG Jing,ZHANG De-chun.Research on Inducement of Lilium lancifolium Protocorm and Plant Regeneration[J].Hubei Agricultural Sciences,2012(2):396-399. |
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| Authors: | HE Wei JING Dan-long CHEN Qiang WANG Jing ZHANG De-chun |
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| Institution: | 1(1.Biotechnology Research Center of China Three Gorges University,Yichang 443002,Hubei,China; 2.Biodiversity Conservation Research Laboratory,Hubei Shennongjia National Nature Reserve Administration,Muyu 442400,Hubei,China) |
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| Abstract: | Regeneration system of Lilium lancifolium was developed using bulbs,leaves and bulbils as explants.The results indicated that bulb was the optimal explant for protocorm induction.The optimum media for callus induction of bulb was MS+1.0 mg/L 6-BA+1.0 mg/L NAA.The optimum media for protocorm was MS+0.5 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L 2,4-D.The compatible budding medium of protocorm was MS+1.0 mg/L 6-BA+0.1 mg/L NAA;And the compatible rooted medium of gemma was MS+0.5 mg/L NAA. |
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| Keywords: | Lilium lancifolium protocorm induction plant regeneration |
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