| 沼液样品静置富集培养及好氧纤维素降解菌的分离 |
| |
| 引用本文： | 程林,严兴,樊美珍,周志华.沼液样品静置富集培养及好氧纤维素降解菌的分离[J].安徽农业大学学报,2008,35(4). |
| |
| 作者姓名： | 程林 严兴 樊美珍 周志华 |
| |
| 作者单位： | 安徽农业大学微生物防治省重点实验室,合肥,230036;上海生命科学院植物生理生态研究所,上海,200032 |
| |
| 基金项目： | 安徽省高校自然科学基金，浙江省湖州市社会主义新农村建设科技专项 |
| |
| 摘 要： | 好氧纤维素降解菌,能够促进沼气系统启动阶段厌氧条件的建立以及木质纤维素的降解,因此对于加速沼气系统产气具有重要的作用.通过沼液的静置富集培养,使用选择性培养基进行分离,分离到了1株适合于沼液环境的好氧纤维素降解菌.对其16S rRNA基因进行测序,结果表明这株菌属于放线菌中的链霉菌属.最后通过克隆该株菌的ERIC-PCR指纹图谱条带,找到了1段该菌株特异性序列,能够为监测该菌在沼气强化过程中的应用提供适合的分子标记.
|
| 关 键 词： | 沼气 好氧纤维素降解菌 ERIC-PCR |
Isolation of aerobic cellulolytic strain from enrichment of biogas slurry |
| |
| CHENG Lin,YAN Xing,FAN Mei-zhen,ZHOU Zhi-hua.Isolation of aerobic cellulolytic strain from enrichment of biogas slurry[J].Journal of Anhui Agricultural University,2008,35(4). |
| |
| Authors: | CHENG Lin YAN Xing FAN Mei-zhen ZHOU Zhi-hua |
| |
| Abstract: | Aerobic cellulolytic bacteria might facilitate formation of anaerobic condition for methanogenesis and degradation of lignocellulosic material at start-up stages of biogas fermentation,so they are important for bioaugmentation of biogas production.In this study,aerobic cellulolytic bacteria were enriched by static culture of biogas slurry and subsequently,one aerobic cellulolytic strain was isolated.The strain belongs to genus of Streptomyces according to 16S rRNA gene sequence.At last,a molecular marker of the strain was obtained by cloning one band of ERIC-PCR fingerprinting,which could be used for monitoring the strain in biogas fermentation. |
| |
| Keywords: | ERIC-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《安徽农业大学学报》浏览原始摘要信息 |
| 点击此处可从《安徽农业大学学报》下载免费的PDF全文 |
|
